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|Title: ||Towards a global barcode library for Lymantria (Lepidoptera: Lymantriinae) tussock moths of biosecurity concern|
|Author: ||Boykin, Laura M.|
Armstrong, Karen F.
deWaard, Jeremy R.
Keena, Melody A.
Pogue, Michael G.
Hanner, Robert H.
Humble, Leland M.
|Date: ||Dec-2010 |
|Publisher: ||Public Library of Science.|
|Citation: ||deWaard, J. R., Mitchell, A., Keena, M. A., Gopurenko, D., Boykin, L. M., Armstrong, K. F., et. al. (2010). Towards a global barcode library for Lymantria (Lepidoptera: Lymantriinae) tussock moths of biosecurity concern. PLoS ONE, 5(12), e14280. doi:10.1371/journal.pone.0014280|
|Item Type: ||Journal Article|
|Abstract: ||Background: Detecting and controlling the movements of invasive species, such as insect pests, relies upon rapid and
accurate species identification in order to initiate containment procedures by the appropriate authorities. Many species in
the tussock moth genus Lymantria are significant forestry pests, including the gypsy moth Lymantria dispar L., and
consequently have been a focus for the development of molecular diagnostic tools to assist in identifying species and
source populations. In this study we expand the taxonomic and geographic coverage of the DNA barcode reference library,
and further test the utility of this diagnostic method, both for species/subspecies assignment and for determination of
geographic provenance of populations.
Methodology/Principal Findings: Cytochrome oxidase I (COI) barcodes were obtained from 518 individuals and 36 species
of Lymantria, including sequences assembled and generated from previous studies, vouchered material in public
collections, and intercepted specimens obtained from surveillance programs in Canada. A maximum likelihood tree was
constructed, revealing high bootstrap support for 90% of species clusters. Bayesian species assignment was also tested, and
resulted in correct assignment to species and subspecies in all instances. The performance of barcoding was also compared
against the commonly employed NB restriction digest system (also based on COI); while the latter is informative for
discriminating gypsy moth subspecies, COI barcode sequences provide greater resolution and generality by encompassing
a greater number of haplotypes across all Lymantria species, none shared between species.
Conclusions/Significance: This study demonstrates the efficacy of DNA barcodes for diagnosing species of Lymantria and
reinforces the view that the approach is an under-utilized resource with substantial potential for biosecurity and
surveillance. Biomonitoring agencies currently employing the NB restriction digest system would gather more information
by transitioning to the use of DNA barcoding, a change which could be made relatively seamlessly as the same gene region
underlies both protocols.|
|Persistent URL (URI): ||http://hdl.handle.net/10182/3668|
|Related: ||Originally published at http://www.plosone.org|
|Related URI: ||http://dx.doi.org/10.1371/journal.pone.0014280|
|Rights: ||This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public
domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.|
|Appears in Collections:||Bio-Protection Research Centre|
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