Volta phase plate cryo-EM of the small protein complex Prx3
Khoshouei, M.; Radjainia, M.; Phillips, Amy; Gerrard, J. A.; Mitra, A. K.; Plitzko, J. M.; Baumeister, W.; Danev, R.
Cryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre resolution cryo-EM structures are rare compared with crystal structure depositions, particularly for relatively small particles (<400 kDa). Here we demonstrate the benefits of Volta phase plates for single-particle analysis by time-efficient cryo-EM structure determination of 257 kDa human peroxiredoxin-3 dodecamers at 4.4 Å resolution. The Volta phase plate improves the applicability of cryo-EM for small molecules and accelerates structure determination.... [Show full abstract]
Fields of Research06 Biological Sciences; 0605 Microbiology; 1007 Nanotechnology; 1001 Agricultural Biotechnology; MD Multidisciplinary
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