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dc.contributor.authorMcKinnon, Aimeeen
dc.contributor.authorGlare, Travisen
dc.contributor.authorRidgway, Hayley J.en
dc.contributor.authorMendoza-Mendoza, A.en
dc.contributor.authorHolyoake, Andrew J.en
dc.contributor.authorGodsoe, Williamen
dc.contributor.authorBufford, Jenniferen
dc.date.accessioned2018-08-02T22:37:08Z
dc.date.issued2018-06-11en
dc.date.submitted2018-05-14en
dc.identifier.citationMcKinnon et al. (2018). Detection of the entomopathogenic fungus Beauveria bassiana in the rhizosphere of wound-stressed Zea mays plants. Frontiers in Microbiology, 9, 1161. doi:10.3389/fmicb.2018.01161en
dc.identifier.issn1664-302Xen
dc.identifier.urihttps://hdl.handle.net/10182/10116
dc.description.abstractEntomopathogenic fungi from the genus Beauveria (Vuillemin) play an important role in controlling insect populations and have been increasingly utilized for the biological control of insect pests. Various studies have reported that Beauveria bassiana (Bals.), Vuill. also has the ability to colonize a broad range of plant hosts as endophytes without causing disease but while still maintaining the capacity to infect insects. Beauveria is often applied as an inundative spore application, but little research has considered how plant colonization may alter the ability to persist in the environment. The aim of this study was to investigate potential interactions between B. bassiana and Zea mays L. (maize) in the rhizosphere following inoculation, in order to understand the factors that may affect environmental persistence of the fungi. The hypothesis was that different isolates of B. bassiana have the ability to colonize maize roots and/or rhizosphere soil, resulting in effects to the plant microbiome. To test this hypothesis, a two-step nested PCR protocol was developed to find and amplify Beauveria in planta or in soil; based on the translation elongation factor 1-alpha (ef1α) gene. The nested protocol was also designed to enable Beauveria species differentiation by sequence analysis. The impact of three selected B. bassiana isolates applied topically to roots on the rhizosphere soil community structure and function were consequently assessed using denaturing gradient gel electrophoresis (DGGE) and MicroResp™ techniques. The microbial community structure and function were not significantly affected by the presence of the isolates, however, retention of the inocula in the rhizosphere at 30 days after inoculation was enhanced when plants were subjected to intensive wounding of foliage to crudely simulate herbivory. The plant defense response likely changed under wound stress resulting in the apparent recruitment of Beauveria in the rhizosphere, which may be an indirect defensive strategy against herbivory and/or the result of induced systemic susceptibility in maize enabling plant colonization.en
dc.format.extent16en
dc.language.isoenen
dc.publisherFrontiers Mediaen
dc.relationThe original publication is available from - Frontiers Media - https://doi.org/10.3389/fmicb.2018.01161en
dc.relation.urihttps://doi.org/10.3389/fmicb.2018.01161en
dc.rights© 2018 McKinnon, Glare, Ridgway, Mendoza-Mendoza, Holyoake, Godsoe and Bufford. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectentomopathogenic fungien
dc.subjectendophytesen
dc.subjectplant interactionen
dc.subjectagroecosystemsen
dc.subjectbiocontrolen
dc.subjectmultitrophic interactionsen
dc.titleDetection of the entomopathogenic fungus Beauveria bassiana in the rhizosphere of wound-stressed Zea mays plantsen
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Pest Management and Conservationen
lu.contributor.unitBio-Protection Research Centreen
dc.identifier.doi10.3389/fmicb.2018.01161en
dc.subject.anzsrc0605 Microbiologyen
dc.subject.anzsrc070303 Crop and Pasture Biochemistry and Physiologyen
dc.subject.anzsrc050303 Soil Biologyen
dc.subject.anzsrc060505 Mycologyen
dc.subject.anzsrc0502 Environmental Science and Managementen
dc.subject.anzsrc0503 Soil Sciencesen
dc.relation.isPartOfFrontiers in Microbiologyen
pubs.notesArticle 1161en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/ECOL
pubs.organisational-group/LU/BPRC
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/QE18
pubs.publication-statusPublisheden
pubs.volume9en
dc.identifier.eissn1664-302Xen
dc.rights.licenceAttributionen
dc.rights.licenceAttributionen
lu.identifier.orcid0000-0003-1697-6916
lu.identifier.orcid0000-0001-7795-8709
lu.identifier.orcid0000-0002-1532-3406
lu.identifier.orcid0000-0003-3320-6024


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