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dc.contributor.authorHe, T.-F.en
dc.contributor.authorZhang, Z.-H.en
dc.contributor.authorZeng, X.-A.en
dc.contributor.authorWang, L.-H.en
dc.contributor.authorBrennan, Charles S.en
dc.date.accessioned2018-08-07T23:32:51Z
dc.date.available2017-11-10en
dc.date.issued2018-01en
dc.date.submitted2017-11-09en
dc.identifier.citationHe, T-F., Zhang, Z-H., Zeng, X-A, Wang, L-H., & Brennan, C.S. (2017). Determination of membrane disruption and genomic DNA binding of cinnamaldehyde to Escherichia coli by use of microbiological and spectroscopic techniques. Journal of Photochemistry and Photobiology, B: Biology, 178, 623-630. doi:10.1016/j.jphotobiol.2017.11.015en
dc.identifier.issn1011-1344en
dc.identifier.urihttps://hdl.handle.net/10182/10122
dc.description.abstractThis work was aimed to investigate the antibacterial action of cinnamaldehyde (CIN) against Escherichia coli ATCC 8735 (E. coli) based on membrane fatty acid composition analysis, alterations of permeability and cell morphology as well as interaction with genomic DNA. Analysis of membrane fatty acids using gas chromatography-mass spectrometry (GC-MS) revealed that the proportion of unsaturated fatty acids (UFA) and saturated fatty acids (SFA) were the major fatty acids in plasmic membrane, and their levels were significantly changed after exposure of E. coli to CIN at low concentrations. For example, the proportion of UFA decreased from 39.97% to 20.98%, while the relative content of SFA increased from 50.14% to 67.80% as E. coli was grown in increasing concentrations of CIN (from 0 to 0.88mM). Scanning electron microscopy (SEM) showed that the morphology of E. coli cells to be wrinkled, distorted and even lysed after exposure to CIN, which therefore decreased the cell viability. The binding of CIN to genomic DNA was probed using fluorescence, UV-Visible absorption spectra, circular dichroism, molecular modeling and atomic force microscopy (AFM). Results indicated that CIN likely bound to the minor groove of genomic DNA, and changed the secondary structure and morphology of this biomacromolecule. Therefore, CIN can be deem as a kind of natural antimicrobial agents, which influence both cell membrane and genomic DNA.en
dc.format.extent623-630en
dc.languageengen
dc.language.isoenen
dc.publisherElsevieren
dc.relationThe original publication is available from - Elsevier - https://doi.org/10.1016/j.jphotobiol.2017.11.015en
dc.relation.urihttps://doi.org/10.1016/j.jphotobiol.2017.11.015en
dc.rights© 2017 Elsevier B.V. All rights reserved.en
dc.subjectcinnamaldehydeen
dc.subjectEscherichia colien
dc.subjectmembrane fatty aciden
dc.subjectgenomic DNAen
dc.subjectBiophysicsen
dc.subject.meshAcroleinen
dc.subject.meshAnti-Bacterial Agentsen
dc.subject.meshBinding Sitesen
dc.subject.meshCell Wallen
dc.subject.meshCircular Dichroismen
dc.subject.meshDNAen
dc.subject.meshFatty Acidsen
dc.subject.meshFatty Acids, Unsaturateden
dc.subject.meshGas Chromatography-Mass Spectrometryen
dc.subject.meshMicroscopy, Atomic Forceen
dc.subject.meshMicroscopy, Electron, Scanningen
dc.subject.meshMolecular Docking Simulationen
dc.subject.meshNucleic Acid Conformationen
dc.subject.meshSpectrophotometry, Ultravioleten
dc.titleDetermination of membrane disruption and genomic DNA binding of cinnamaldehyde to Escherichia coli by use of microbiological and spectroscopic techniquesen
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Wine, Food and Molecular Biosciencesen
lu.contributor.unitResearch Management Officeen
lu.contributor.unit/LU/Research Management Office/2018 PBRF Staff groupen
dc.identifier.doi10.1016/j.jphotobiol.2017.11.015en
dc.subject.anzsrc0908 Food Sciencesen
dc.subject.anzsrc0601 Biochemistry And Cell Biologyen
dc.subject.anzsrc0299 Other Physical Sciencesen
dc.relation.isPartOfJournal of Photochemistry and Photobiology, B: Biologyen
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/WFMB
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/2018 PBRF Staff group
pubs.publication-statusPublished onlineen
pubs.volume178en
dc.identifier.eissn1873-2682en
lu.identifier.orcid0000-0003-2479-8478


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