The Ustilago maydis repetitive effector Rsp3 blocks the antifungal activity of mannose-binding maize proteins
Ma, L.-S.; Wang, L.; Trippel, C.; Mendoza-Mendoza, A.; Ullmann, S.; Moretti, M.; Carsten, A.; Kahnt, J.; Reissmann, S.; Zechmann, B.; Bange, G.; Kahmann, R.
To cause disease in maize, the biotrophic fungus Ustilago maydis secretes a large arsenal of effector proteins. Here, we functionally characterize the repetitive effector Rsp3 (repetitive secreted protein 3), which shows length polymorphisms in field isolates and is highly expressed during biotrophic stages. Rsp3 is required for virulence and anthocyanin accumulation. During biotrophic growth, Rsp3 decorates the hyphal surface and interacts with at least two secreted maize DUF26-domain family proteins (designated AFP1 and AFP2). AFP1 binds mannose and displays antifungal activity against the rsp3 mutant but not against a strain constitutively expressing rsp3. Maize plants silenced for AFP1 and AFP2 partially rescue the virulence defect of rsp3 mutants, suggesting that blocking the antifungal activity of AFP1 and AFP2 by the Rsp3 effector is an important virulence function. Rsp3 orthologs are present in all sequenced smut fungi, and the ortholog from Sporisorium reilianum can complement the rsp3 mutant of U. maydis, suggesting a novel widespread fungal protection mechanism.... [Show full abstract]
Keywordsmaize; pathogens; Rsp3; fungus; proteins; Ustilago maydis; Ustilago; Plants, Genetically Modified; Zea mays; Mannose; Fungal Proteins; Plant Proteins; Virulence Factors; Gene Expression Profiling; Virulence; Plant Diseases; Gene Silencing; Genome, Fungal; Host-Pathogen Interactions
Fields of Research0605 Microbiology; 100202 Biological Control; 060704 Plant Pathology; 070603 Horticultural Crop Protection (Pests, Diseases and Weeds)
© The Author(s) 2018. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License.Attribution 4.0 International
CitationMa et al. (2018). The Ustilago maydis repetitive effector Rsp3 blocks the antifungal activity of mannose-binding maize proteins. Nature Communications, 9, 1711. doi:10.1038/s41467-018-04149-0
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