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dc.contributor.authorVargas, M. L.en
dc.contributor.authorCruickshank, Robert H.en
dc.contributor.authorRoss, James G.en
dc.contributor.authorHolyoake, Andrew J.en
dc.contributor.authorOgilvie, Shaun C.en
dc.contributor.authorPaterson, Adrian M.en
dc.date.accessioned2009-04-28T02:18:41Z
dc.date.available2020-12-02T11:30:21Z
dc.date.issued2009-01-16en
dc.identifier.citationVargas, M. L., Cruickshank, R. H., Ross, J. G., Holyoake, A. J., Ogilvie, S. C., & Paterson, A. M. (2009). Noninvasive recovery and detection of possum Trichosurus vulpecula DNA from bitten bait interference devices (WaxTags). Molecular Ecology Resources, 9(2), 505-515.en
dc.identifier.issn1755-0998en
dc.identifier.urihttps://hdl.handle.net/10182/1018
dc.description.abstractThe brushtail possum is a major agricultural and ecological pest in New Zealand. A novel noninvasive DNA sampling tool for detecting its presence (WaxTags, or WT) was tested. DNA was recovered from saliva left on WT, and two lengths (407 bp and 648 bp) of the cytochrome c oxidase I (COI) barcoding region were amplified by polymerase chain reaction (PCR). PCR products were considered (+) when a DNA band was clearly visible by electrophoresis. Different factors that might affect PCR (+) were investigated with captive possums: (i) both extraction protocols of the QIAGEN DNeasy Blood and Tissue Kit, (ii) effect of an overnight or longer delay of up to 3 weeks before DNA extraction on both COI amplicons, and (iii) effect of the individual, order and magnitude of the bite. Extraction protocols were not significantly different. The effect of the overnight delay was not significant, and amplification of the short amplicon was significantly higher (100%) than for the long fragment (48%). After a two or 3-week delay, the short amplicon had 94% and 56% PCR (+), success rates, respectively. Individual, order and magnitude of a bite had no significant effect. The delay trial was repeated with WT from the wild, for which PCR (+) rate of the short amplicon was 63%, regardless of freshness. Four microsatellites were amplified from captive WT samples. We conclude that DNA from saliva traces can be recovered from WT, a potential new tool for noninvasive monitoring of possums and other wildlife.en
dc.format.extent505-515en
dc.language.isoenen
dc.publisherWileyen
dc.relationThe original publication is available from - Wiley - https://doi.org/10.1111/j.1755-0998.2008.02498.x - http://hdl.handle.net/10182/1018en
dc.relation.urihttps://doi.org/10.1111/j.1755-0998.2008.02498.xen
dc.rights© 2009 Blackwell Publishing Ltden
dc.subjectDNAen
dc.subjectforensicsen
dc.subjectmonitoringen
dc.subjectsalivaen
dc.subjectTrichosurus vulpeculaen
dc.subjectpossumen
dc.subjectpossumen
dc.subjectEvolutionary Biologyen
dc.titleNoninvasive recovery and detection of possum Trichosurus vulpecula DNA from bitten bait interference devices (WaxTags)en
dc.typeJournal Article
dc.subject.marsdenFields of Research::270000 Biological Sciences::270200 Geneticsen
dc.subject.marsdenFields of Research::300000 Agricultural, Veterinary and Environmental Sciences::300800 Environmental Science::300802 Wildlife and habitat managementen
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Pest Management and Conservationen
lu.contributor.unitBio-Protection Research Centreen
dc.identifier.doi10.1111/j.1755-0998.2008.02498.xen
dc.subject.anzsrc06 Biological Sciencesen
dc.relation.isPartOfMolecular ecology resourcesen
pubs.issue2en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/ECOL
pubs.organisational-group/LU/BPRC
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/PE20
pubs.organisational-group/LU/Research Management Office/QE18
pubs.publication-statusPublisheden
pubs.publisher-urlhttp://hdl.handle.net/10182/1018en
pubs.volume9en
lu.identifier.orcid0000-0001-7413-4704
lu.identifier.orcid0000-0003-4090-0815


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