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dc.contributor.authorLau, Yii Chungen
dc.contributor.authorMorton, James D.en
dc.contributor.authorDeb-Choudhury, S.en
dc.contributor.authorClerens, S.en
dc.contributor.authorDyer, J. M.en
dc.contributor.authorRamli, U. S.en
dc.date.accessioned2019-01-11T02:49:22Z
dc.date.issued2017-03en
dc.date.submitted2017-01-13en
dc.identifier.citationLau et al. (2017). Differential expression analysis of oil palm fatty acid biosynthetic enzymes with gel-free quantitative proteomics. Journal of Oil Palm Research, 29(1), 23-34.en
dc.identifier.issn1511-2780en
dc.identifier.urihttps://hdl.handle.net/10182/10434
dc.description.abstractThe control mechanisms of de novo fatty acid biosynthesis continue to intrigue, particularly at protein level. The cross talk between transcriptional and metabolic regulatory networks remains undefined. In order to understand the metabolic factors affecting oleic acid biosynthesis in Elaeis guineensis var. tenera (oil palm), two-dimensional liquid chromatography-tandem mass spectrometry (2DLC-MS/ MS) was attempted to capture the main fatty acid biosynthetic enzymes involved in oleic acid production. These enzymes were extracted from the chromoplast of low and high oleic mesocarps of 20th week after anthesis. Chromoplast proteins were separated using offline 2DLC for mass spectrometric analysis. Two subunits of acetyl-CoA carboxylase, beta-ketoacyl-acyl carrier protein (ACP) reductase, beta-hydroxyacyl-ACP dehydrogenase, 3-enoylACP reductase, beta-ketoacyl-ACP synthase and stearoyl-ACP desaturase were identified. The relative expression of these fatty acid biosynthetic enzymes was determined using isobaric tags labelling. Initial assessment revealed that beta-hydroxyacyl-ACP dehydrogenase and beta-ketoacyl-ACP synthase were present in a higher abundance in the high oleic acid mesocarps while the accumulation of 3-enoyl-ACP reductase was lower relative to the other fatty acid biosynthetic enzymes investigated. The existence of other differentially expressed metabolic enzymes suggests that the control mechanism of fatty acid production, in particular oleic acid, might involve more than just the main fatty acid biosynthetic enzymes.en
dc.format.extent23-34en
dc.language.isoenen
dc.publisherMalaysian Palm Oil Boarden
dc.relationThe original publication is available from - Malaysian Palm Oil Board - http://jopr.mpob.gov.my/differential-expression-analysis-of-oil-palm-fatty-acid-biosynthetic-enzymes-with-gel-free-quantitative-proteomics/?v=trueen
dc.rights© 2017 Malaysian Palm Oil Board. All Rights Reserved.en
dc.subjectprotein expressionen
dc.subjectfatty acid biosynthesisen
dc.subjectproteomicsen
dc.titleDifferential expression analysis of oil palm fatty acid biosynthetic enzymes with gel-free quantitative proteomicsen
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Wine, Food and Molecular Biosciencesen
lu.contributor.unitResearch Management Officeen
lu.contributor.unit/LU/Research Management Office/2018 PBRF Staff groupen
dc.subject.anzsrc060702 Plant Cell and Molecular Biologyen
dc.subject.anzsrc060799 Plant Biology Not Elsewhere Classifieden
dc.relation.isPartOfJournal of Oil Palm Researchen
pubs.issue1en
pubs.notesNo DOI availableen
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/WFMB
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/2018 PBRF Staff group
pubs.publication-statusPublisheden
pubs.publisher-urlhttp://jopr.mpob.gov.my/differential-expression-analysis-of-oil-palm-fatty-acid-biosynthetic-enzymes-with-gel-free-quantitative-proteomics/?v=trueen
pubs.volume29en
lu.identifier.orcid0000-0001-9645-5568


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