The interactions of iron nutrition, salinity and ultraviolet-B radiation on the physiological responses of wheat (Triticum aestivum L.)

Abstract

Increasing human population, land degradation and global change is intensifying pressure on agricultural production in marginal environments. Many marginal environments experience multiple stressors, however, few studies have investigated the interaction of more than two stressors. This is the first study examining the interaction of Fe nutrition, salinity and ultraviolet-B (UV-B) radiation in plants. Wheat (Triticum aestivum L. cv. 1862) was grown in a three-way factorial design: iron (75 µM Fe and 3.75 µM Fe as FeCl₃), salinity (0 mM and 75 mM NaCl) and UV-B radiation (1 kJ m¯² d¯¹, and 15 kJ m¯² d¯¹ biologically effective UV-B) in chelator-buffered solutions. Examination of the stress interactions showed that phytosiderophore release and root Fe accumulation were limited by UV-B and NaCl stress under Fe deficiency. The findings demonstrated Fe dependency of plant responses to salinity and to UV-B, e.g. sufficient Fe is required for UV-B-induced stomatal opening. Other interactions included that elevated salinity alleviated Fe deficiency-induced decreases in water use efficiency. In addition, Fe deficiency increased the accumulation of UV-absorbing compounds under elevated UV-B radiation. UV-B radiation affected belowground morphology and physiology, including a 47 % reduction in phytosiderophore release. This is the first study to demonstrate a role for wavelengths other than photosynthetically active radiation in phytosiderophore release. Our findings demonstrate that aboveground environmental factors such as UV-B radiation can affect important aspects of belowground plant function, and that Fe availability needs to be considered when growing plants in saline or high UV-B radiation environments.