A study on the uptake of plant hormones into protoplasts: A report submitted in partial fulfilment of the requirement for the degree of B.Sc. (Hons) in the University of Canterbury

Abstract

The following experiments were done with the aim of developing a technique of finding out whether or not auxin stimulates the uptake of radioactivelly labelled gibberellin into plant cells. Experiments in this area in the past have used whole plants and portions of plant tissue. In this experiment we have attempted to use a relatively homogenous population of cells by the isolation of cells from a leaf. Protoplasts were used instead of cells because this eliminated a possible problem of the radioactivelly labelled hormone being adsorbed onto the cell wall.