Hierarchical cluster analysis of herbicide modes of action reveals distinct classes of multiple resistance in weeds

Abstract

BACKGROUND: The number of weed species resistant to multiple herbicide modes of action (MoAs) has increased over the last 30 years and may in the future render existing herbicide MoAs obsolete for many cropping systems. Yet few predictive tools exist to manage this risk. Using a worldwide dataset of weed species resistant to multiple herbicide MoAs, hierarchical clustering was used to classify MoAs into similar groups in relation to the suite of resistant weed species they have in common. Network analyses then were used to explore the relative importance of species prevalence and similarity in cluster patterns. RESULTS: Hierarchical clustering identified three similarly sized clusters of herbicide MoAs that were linked by the co-occurrence of resistant weeds: Herbicide Resistance Action Committee (HRAC) groups 2, 4, 5 and 9; HRAC groups 12, 14 and 15; and HRAC groups 1, 3 and 22. Cluster membership was consistent with similarities in the physiological or biochemical target of the herbicide MoAs. Network analyses revealed that the number of weed species resistant to two different MoAs was related to the number of weeds known to be resistant to each individual herbicide MoA. CONCLUSIONS: Hierarchical cluster analysis provided new insights into the risk of weeds becoming resistant to more than one herbicide MoA. By clustering herbicide MoAs into three distinct groups, the potential exists for farmers to manage resistance by rotating herbicides between rather than within clusters, as far as crop, weed and environmental conditions allow.