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dc.contributor.authorSu, H-Y
dc.contributor.authorHickford, Jonathan
dc.contributor.authorThe, PHB
dc.contributor.authorHill, AM
dc.contributor.authorFrampton, CM
dc.contributor.authorBickerstaffe, Roy
dc.coverage.spatialUnited States
dc.date.accessioned2018-08-16T22:28:52Z
dc.date.available2015-12-08
dc.date.issued1999-02
dc.date.submitted1998-10-15
dc.identifierS0022-202X(15)40405-1
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000078287000017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=42fe17854fe8be72a22db98beb5d2208
dc.identifier.issn0022-202X
dc.identifier.other9989803 (pubmed)
dc.identifier.urihttps://hdl.handle.net/10182/10146
dc.description.abstractInsulin-like growth factor 1 (IGF-1) mediates many of the actions of growth hormone. Overexpression of IGF-1 has been reported to have endocrine and paracrine/autocrine effects on somatic growth in transgenic mice. To study the paracrine/autocrine effects of IGF-1 in hair follicles, transgenic mice were produced by pronuclear microinjection of a construct containing a mouse ultra-high sulfur keratin (UHS-KER) promoter linked to an ovine IGF-1 cDNA. This UHS-KER promoter has previously been shown to direct expression of a reporter gene to the hair follicles of transgenic mice. Four transgenic mouse lines were established as a result of microinjection of 435 embryos. Transgene expression was found in skin at day 8 and day 15 of age in three of the lines. Progeny tests were carried out by mating two of the transgenic expressing males to nontransgenic females. Mice from one line were all nonexpressors while four of the 12 mice from the other showed integration of the transgene and three expressed transgene IGF-1 mRNA in the skin. Vibrissa growth at 11-21 d of age was significantly greater in transgenic expressors than in their nontransgenic littermates. Specifically, the increase in vibrissa length for transgenics at days 11-16 (20.5%) is ≃2-fold compared with days 16-21 (11.9%). These results demonstrate that local overexpression of IGF-1 in transgenic mice is capable of stimulating vibrissa growth during the first neonatal hair cycle.
dc.description.sponsorshipThe work was supported in part by the University Research Trust of Lincoln University.
dc.format.extentpp.245-248
dc.format.mediumPrint
dc.languageen
dc.language.isoen
dc.publisherElsevier on behalf of the Society for Investigative Dermatology, Inc.
dc.relationThe original publication is available from Elsevier on behalf of the Society for Investigative Dermatology, Inc. - https://doi.org/10.1046/j.1523-1747.1999.00489.x
dc.relation.urihttps://doi.org/10.1046/j.1523-1747.1999.00489.x
dc.rights© 1999 by The Society for Investigative Dermatology, Inc. All rights reserved.
dc.subjectinsulin-like growth factor 1 (IGF-1)
dc.subjecttransgenic mice
dc.subjectendocrine
dc.subjectparacrine
dc.subjectneonatal hair cycle
dc.subjectvibrissa growth
dc.subjectautocrine
dc.subject.meshVibrissae
dc.subject.meshAnimals
dc.subject.meshMice, Transgenic
dc.subject.meshMice
dc.subject.meshBody Weight
dc.subject.meshCystine
dc.subject.meshSulfur Radioisotopes
dc.subject.meshInsulin-Like Growth Factor I
dc.subject.meshRNA, Messenger
dc.subject.meshBlotting, Southern
dc.subject.meshGene Expression
dc.subject.meshAnimals
dc.subject.meshBlotting, Southern
dc.subject.meshBody Weight
dc.subject.meshCystine
dc.subject.meshGene Expression
dc.subject.meshInsulin-Like Growth Factor I
dc.subject.meshMice
dc.subject.meshMice, Transgenic
dc.subject.meshRNA, Messenger
dc.subject.meshSulfur Radioisotopes
dc.subject.meshVibrissae
dc.titleIncreased vibrissa growth in transgenic mice expressing insulin-like growth factor 1
dc.typeJournal Article
lu.contributor.unitLincoln University
lu.contributor.unitFaculty of Agriculture and Life Sciences
lu.contributor.unitDepartment of Agricultural Sciences
lu.contributor.unitDepartment of Wine, Food and Molecular Biosciences
dc.identifier.doi10.1046/j.1523-1747.1999.00489.x
dc.subject.anzsrc110107 Metabolic Medicine
dc.subject.anzsrc11 Medical and Health Sciences
dc.subject.anzsrc110304 Dermatology
dc.relation.isPartOfJournal of Investigative Dermatology
pubs.issue2
pubs.organisational-group|LU
pubs.organisational-group|LU|Agriculture and Life Sciences
pubs.organisational-group|LU|Agriculture and Life Sciences|AGSC
pubs.organisational-group|LU|Agriculture and Life Sciences|WFMB
pubs.organisational-group|LU|Research Management Office
pubs.organisational-group|LU|Research Management Office|QE18
pubs.publication-statusPublished
pubs.volume112
dc.identifier.eissn1523-1747
lu.identifier.orcid0000-0001-9313-761X


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