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dc.contributor.authorZhao, Menglien
dc.contributor.authorZhou, Huitongen
dc.contributor.authorHickford, Jonathan G. H.en
dc.contributor.authorGong, Huaen
dc.contributor.authorWang, Jiqingen
dc.contributor.authorHu, Jiangen
dc.contributor.authorLiu, Xiuen
dc.contributor.authorLi, Shaobinen
dc.contributor.authorHao, Zhiyunen
dc.contributor.authorLuo, Yuzhuen
dc.date.accessioned2019-05-03T03:59:54Z
dc.date.available2019-03-26en
dc.date.issued2019-03-26en
dc.date.submitted2019-03-05en
dc.identifier.citationZhao, M., Zhou, H., Hickford, J. G. H., Gong, H., Wang, J., Hu, J., . . . Luo, Y. (2019). Variation in the caprine keratin-associated protein 15-1 (KAP15-1) gene affects cashmere fibre diameter. Archives Animal Breeding, 62(1), 125-133. doi:10.5194/aab-62-125-2019en
dc.identifier.issn0003-9438en
dc.identifier.urihttps://hdl.handle.net/10182/10625
dc.description.abstractKeratin-associated proteins (KAPs) are a structural component of cashmere fibre, and variation in some KAP genes (KRTAPs) has been associated with a number of caprine fibre traits. In this study, we report the identification of KRTAP15-1 in goats. Sequence variation in the gene was detected using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) technique in 250 Longdong goats, and six variants (named A to F) containing eight single nucleotide polymorphisms (SNPs) were identified. Five of the SNPs were non-synonymous and would lead to putative amino acid changes. Reverse-transcription polymerase chain reaction (RT-PCR) analysis revealed that KRTAP15-1 was expressed in secondary hair follicles but not in heart tissue, liver tissue, lung tissue, kidney tissue or the longissimus dorsi muscle. Despite being rich in cysteine, the caprine KAP15-1 protein possesses a high content of serine and moderate content of glycine and phenylalanine. Association analyses revealed that KRTAP15-1 variant A was associated with decreased mean fibre diameter (MFD), and this effect appeared to be dominant; while variant C was found to be associated with increased MFD, the effect being recessive. The findings suggest that caprine KRTAP15-1 is highly polymorphic and that variation in this gene affects cashmere MFD.en
dc.format.extent125-133en
dc.language.isoenen
dc.publisherCopernicus Publications on behalf of the Leibniz Institute for Farm Animal Biologyen
dc.relationThe original publication is available from - Copernicus Publications on behalf of the Leibniz Institute for Farm Animal Biology - https://doi.org/10.5194/aab-62-125-2019en
dc.relation.urihttps://doi.org/10.5194/aab-62-125-2019en
dc.rights© 2019 Author(s).en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectkeratinen
dc.subjectproteinen
dc.subjectcashmere fibreen
dc.subjectDairy & Animal Scienceen
dc.titleVariation in the caprine keratin-associated protein 15-1 (KAP15-1) gene affects cashmere fibre diameteren
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Agricultural Sciencesen
dc.identifier.doi10.5194/aab-62-125-2019en
dc.subject.anzsrc0702 Animal Productionen
dc.subject.anzsrc0601 Biochemistry and Cell Biologyen
dc.subject.anzsrc0701 Agriculture, Land and Farm Managementen
dc.relation.isPartOfArchives Animal Breedingen
pubs.issue1en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/AGSC
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/QE18
pubs.publication-statusPublisheden
pubs.volume62en
dc.identifier.eissn2363-9822en
dc.rights.licenceAttributionen
dc.rights.licenceAttributionen
lu.identifier.orcid0000-0001-9313-761X
lu.identifier.orcid0000-0002-2247-3824
lu.identifier.orcid0000-0001-7620-3160


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