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dc.contributor.authorZhao, Menglien
dc.contributor.authorZhou, Huitongen
dc.contributor.authorHickford, Jonathan G. H.en
dc.contributor.authorGong, Huaen
dc.contributor.authorWang, Jiqingen
dc.contributor.authorHu, Jiangen
dc.contributor.authorLiu, Xiuen
dc.contributor.authorLi, Shaobinen
dc.contributor.authorHao, Zhiyunen
dc.contributor.authorLuo, Yuzhuen
dc.identifier.citationZhao, M., Zhou, H., Hickford, J. G. H., Gong, H., Wang, J., Hu, J., . . . Luo, Y. (2019). Variation in the caprine keratin-associated protein 15-1 (KAP15-1) gene affects cashmere fibre diameter. Archives Animal Breeding, 62(1), 125-133. doi:10.5194/aab-62-125-2019en
dc.description.abstractKeratin-associated proteins (KAPs) are a structural component of cashmere fibre, and variation in some KAP genes (KRTAPs) has been associated with a number of caprine fibre traits. In this study, we report the identification of KRTAP15-1 in goats. Sequence variation in the gene was detected using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) technique in 250 Longdong goats, and six variants (named A to F) containing eight single nucleotide polymorphisms (SNPs) were identified. Five of the SNPs were non-synonymous and would lead to putative amino acid changes. Reverse-transcription polymerase chain reaction (RT-PCR) analysis revealed that KRTAP15-1 was expressed in secondary hair follicles but not in heart tissue, liver tissue, lung tissue, kidney tissue or the longissimus dorsi muscle. Despite being rich in cysteine, the caprine KAP15-1 protein possesses a high content of serine and moderate content of glycine and phenylalanine. Association analyses revealed that KRTAP15-1 variant A was associated with decreased mean fibre diameter (MFD), and this effect appeared to be dominant; while variant C was found to be associated with increased MFD, the effect being recessive. The findings suggest that caprine KRTAP15-1 is highly polymorphic and that variation in this gene affects cashmere MFD.en
dc.publisherCopernicus Publications on behalf of the Leibniz Institute for Farm Animal Biologyen
dc.relationThe original publication is available from - Copernicus Publications on behalf of the Leibniz Institute for Farm Animal Biology -
dc.rights© 2019 Author(s).en
dc.subjectcashmere fibreen
dc.subjectDairy & Animal Scienceen
dc.titleVariation in the caprine keratin-associated protein 15-1 (KAP15-1) gene affects cashmere fibre diameteren
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Agricultural Sciencesen
dc.subject.anzsrc0702 Animal Productionen
dc.subject.anzsrc0601 Biochemistry and Cell Biologyen
dc.subject.anzsrc0701 Agriculture, Land and Farm Managementen
dc.relation.isPartOfArchives Animal Breedingen
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/AGSC
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/QE18

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