Identification of ovine KRTAP28-1 and its association with wool fibre diameter
Keratin-associated proteins (KAPs) are a diverse group of proteins and form a matrix that cross-links keratin intermediate filaments in hair and wool fibres. From over 100 KAP genes (KRTAPs) identified in mammalian species, KRTAP25-1 is a high sulphur (HS)-KAP gene, which has recently been described in humans. Here, we report the absence of KRTAP25-1 in sheep, and describe a new HS-KRTAP (named KRTAP28-1) in the chromosome region where KRTAP25-1 was expected to be found. Six variants (A−F) of KRTAP28-1 containing eight single nucleotide polymorphisms (SNPs) and a TG repeat polymorphism were detected. One was positioned 30 bp upstream of the transcription start codon and all the others were non-synonymous SNPs, including a nonsense SNP. The TG repeat polymorphism would lead to a reading frame shift at the carboxyl-terminal end. The effect of KRTAP28-1 on wool traits was investigated with 383 Southdown × Merino-cross lambs from seven sire lines. Of the four genotypes with a frequency of over 5%, lambs of genotypes AB and BD produced wool of a smaller MFD than lambs of genotype BC. This shows that KRTAP28-1 is associated with wool fibre diameter, and that variation in this gene might have potential for use as a gene marker for reducing wool fibre diameter.... [Show full abstract]
Keywordssheep; keratin-associated protein KAP28-1 gene (KRTAP28-1); polymerase chain reaction (PCR); single-stranded conformational polymorphism (SSCP); variation; wool fibre diameter
Fields of Research0702 Animal Production; 070201 Animal Breeding; 0604 Genetics; 060403 Developmental Genetics (incl. Sex Determination); 060411 Population, Ecological and Evolutionary Genetics; 060412 Quantitative Genetics (incl. Disease and Trait Mapping Genetics); 0502 Environmental Science and Management; 0608 Zoology
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