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dc.contributor.authorBai, Lingrongen
dc.contributor.authorWang, Jingen
dc.contributor.authorZhou, Huitongen
dc.contributor.authorGong, Huaen
dc.contributor.authorTao, Jinzhongen
dc.contributor.authorHickford, Jonathan G. H.en
dc.date.accessioned2019-06-27T00:06:52Z
dc.date.available2019-04-02en
dc.date.issued2019-04en
dc.date.submitted2019-03-29en
dc.identifier.citationBai et al. (2019). Identification of ovine KRTAP28-1 and its association with wool fibre diameter. Animals, 9(4), 142. doi:10.3390/ani9040142en
dc.identifier.issn2076-2615en
dc.identifier.urihttps://hdl.handle.net/10182/10788
dc.description.abstractKeratin-associated proteins (KAPs) are a diverse group of proteins and form a matrix that cross-links keratin intermediate filaments in hair and wool fibres. From over 100 KAP genes (KRTAPs) identified in mammalian species, KRTAP25-1 is a high sulphur (HS)-KAP gene, which has recently been described in humans. Here, we report the absence of KRTAP25-1 in sheep, and describe a new HS-KRTAP (named KRTAP28-1) in the chromosome region where KRTAP25-1 was expected to be found. Six variants (A−F) of KRTAP28-1 containing eight single nucleotide polymorphisms (SNPs) and a TG repeat polymorphism were detected. One was positioned 30 bp upstream of the transcription start codon and all the others were non-synonymous SNPs, including a nonsense SNP. The TG repeat polymorphism would lead to a reading frame shift at the carboxyl-terminal end. The effect of KRTAP28-1 on wool traits was investigated with 383 Southdown × Merino-cross lambs from seven sire lines. Of the four genotypes with a frequency of over 5%, lambs of genotypes AB and BD produced wool of a smaller MFD than lambs of genotype BC. This shows that KRTAP28-1 is associated with wool fibre diameter, and that variation in this gene might have potential for use as a gene marker for reducing wool fibre diameter.en
dc.format.extent11en
dc.language.isoenen
dc.publisherMDPIen
dc.relationThe original publication is available from - MDPI - https://doi.org/10.3390/ani9040142en
dc.relation.urihttps://doi.org/10.3390/ani9040142en
dc.rights© The authors. Licensee MDPI, Basel, Switzerland.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectsheepen
dc.subjectkeratin-associated protein KAP28-1 gene (KRTAP28-1)en
dc.subjectpolymerase chain reaction (PCR)en
dc.subjectsingle-stranded conformational polymorphism (SSCP)en
dc.subjectvariationen
dc.subjectwool fibre diameteren
dc.titleIdentification of ovine KRTAP28-1 and its association with wool fibre diameteren
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Agricultural Sciencesen
lu.contributor.uniten
lu.contributor.uniten
dc.identifier.doi10.3390/ani9040142en
dc.subject.anzsrc0702 Animal Productionen
dc.subject.anzsrc070201 Animal Breedingen
dc.subject.anzsrc0604 Geneticsen
dc.subject.anzsrc060403 Developmental Genetics (incl. Sex Determination)en
dc.subject.anzsrc060411 Population, Ecological and Evolutionary Geneticsen
dc.subject.anzsrc060412 Quantitative Genetics (incl. Disease and Trait Mapping Genetics)en
dc.relation.isPartOfAnimalsen
pubs.issue4en
pubs.notesArticle ID 142en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/AGSC
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/2018 PBRF Staff group
pubs.publication-statusPublisheden
pubs.volume9en
dc.identifier.eissn2076-2615en
dc.rights.licenceAttributionen
dc.rights.licenceAttributionen
lu.identifier.orcid0000-0001-7620-3160
lu.identifier.orcid0000-0002-2247-3824
lu.identifier.orcid0000-0001-9313-761X


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