Show simple item record

dc.contributor.authorGong, Hua
dc.contributor.authorZhou, Huitong
dc.contributor.authorBai, Lingrong
dc.contributor.authorLi, W.
dc.contributor.authorLi, S.
dc.contributor.authorWang, J.
dc.contributor.authorLuo, Y.
dc.contributor.authorHickford, Jonathan G. H.
dc.date.accessioned2019-07-03T00:59:12Z
dc.date.available2018-12-08en
dc.date.issued2019-02
dc.date.submitted2018-12-06en
dc.identifier.issn0021-8812en
dc.identifier.urihttps://hdl.handle.net/10182/10807
dc.description.abstractThe keratin-associated proteins (KAPs) are important constituents of wool fibres. Of the many mammalian KAP genes (KRTAPs) identified, KRTAP20-1 has been described in humans, but it has not been described in any other species. A search of the sheep genome using the human KRTAP20-1 sequence revealed a homologous open reading frame on chromosome 1, which would encode a high glycine-tyrosine KAP. PCR-single stranded conformational polymorphism (PCR-SSCP) analysis identified eight different banding patterns representing eight unique DNA sequences (named A-H). The sequences had highest similarity to the human KRTAP20-1 sequence, and this suggests they are variants of ovine KRTAP20-1. Among these variants, a 12-bp insertion/deletion and six single nucleotide polymorphisms (SNPs) including one 5' untranslated region (UTR) SNP, one 3' UTR SNP and two non-synonymous SNPs, were detected. Variant A was found to be associated with a decrease in mean fibre diameter, fibre diameter standard deviation and prickle factor; while variant C was associated with increased greasy fleece weight and decreased wool yield. These associations persisted after adjusting for the effect of two nearby KRTAPs (KRTAP6-3 and KRTAP22-1) that have also been reported to associate with these wool traits. This suggests that variation in KRTAP20-1 affects wool yield and mean fibre diameter-associated traits, and that this effect is unlikely to be the result of the clustering of these KRTAPs on chromosome 1.en
dc.format.extent587-595en
dc.languageengen
dc.language.isoen
dc.publisherOxford University Press on behalf of American Society of Animal Science
dc.relationThe original publication is available from - Oxford University Press on behalf of American Society of Animal Science - https://doi.org/10.1093/jas/sky465en
dc.relation.urihttps://doi.org/10.1093/jas/sky465en
dc.rights© The Author(s) 2018. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved.
dc.subjectkeratin-associated protein KAP20-1 gene ( KRTAP20-1 )en
dc.subjectmean fiber diameteren
dc.subjectsheepen
dc.subjectvariationen
dc.subjectwool yielden
dc.subjectkeratin-associated protein KAP20-1 gene (KRTAP20-1)en
dc.subjectDairy & Animal Scienceen
dc.subject.meshWoolen
dc.subject.meshAnimalsen
dc.subject.meshTyrosineen
dc.subject.meshGlycineen
dc.subject.meshPolymerase Chain Reactionen
dc.subject.meshSequence Alignmenten
dc.subject.meshPhylogenyen
dc.subject.meshBase Sequenceen
dc.subject.meshPhenotypeen
dc.subject.meshPolymorphism, Single-Stranded Conformationalen
dc.subject.meshPolymorphism, Single Nucleotideen
dc.subject.meshGenomeen
dc.subject.meshKeratinsen
dc.titleAssociations between variation in the ovine high glycine-tyrosine keratin-associated protein gene KRTAP20-1 and wool traitsen
dc.typeJournal Article
lu.contributor.unitLincoln University
lu.contributor.unitFaculty of Agriculture and Life Sciences
lu.contributor.unitDepartment of Agricultural Sciences
dc.identifier.doi10.1093/jas/sky465en
dc.relation.isPartOfJournal of Animal Scienceen
pubs.issue2en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/AGSC
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/QE18
pubs.publication-statusPublisheden
pubs.volume97en
dc.identifier.eissn1525-3163en
lu.identifier.orcid0000-0001-7620-3160
lu.identifier.orcid0000-0001-9313-761X
lu.identifier.orcid0000-0002-2247-3824


Files in this item

Default Thumbnail

This item appears in the following Collection(s)

Show simple item record