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dc.contributor.authorAsad, Muhammaden
dc.contributor.authorMartoni, Francescoen
dc.contributor.authorRoss, James G.en
dc.contributor.authorWaseem, M.en
dc.contributor.authorAbbas, Fakhar-ien
dc.contributor.authorPaterson, Adrian M.en
dc.date.accessioned2019-07-31T04:54:48Z
dc.date.available2019-07-16en
dc.date.issued2019-07-16en
dc.date.submitted2019-06-03en
dc.identifier.citationAsad et al. (2019). Assessing subspecies status of leopards (Panthera pardus) of northern Pakistan using mitochondrial DNA. PeerJ, 7, e7243. doi:10.7717/peerj.7243en
dc.identifier.issn2167-8359en
dc.identifier.urihttps://hdl.handle.net/10182/10848
dc.description.abstractDespite being classified as critically endangered, little work has been done on leopard protection in Pakistan. Once widely present throughout this region, leopards are now sparsely distributed, and possibly extinct from much of their previously recorded habitat. While leopards show morphological and genetic variation across their species range worldwide, resulting in the classification of nine different subspecies, the leopard genetic structure across Pakistan is unknown, with previous studies including only a very limited sampling. To clarify the genetic status of leopards in Pakistan we investigated the sequence variation in the subunit 5 of the mitochondrial gene NADH from 43 tissue samples and compared it with 238 sequences available from online databases. Phylogenetic analysis clearly separates the Pakistani leopards from the African and Arabian clades, confirming that leopards from Pakistan are members of the Asian clade. Furthermore, we identified two separate subspecies haplotypes within our dataset: P. p. fusca (N = 23) and P. p. saxicolor (N = 12).en
dc.format.extent14en
dc.languageenen
dc.language.isoenen
dc.publisherPeerJen
dc.relationThe original publication is available from - PeerJ - https://doi.org/10.7717/peerj.7243en
dc.relation.urihttps://doi.org/10.7717/peerj.7243en
dc.rights© 2019 Asad et al.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectgenetic variationen
dc.subjectnew haplotypeen
dc.subjectdistributionen
dc.subjectPanthera pardus saxicoloren
dc.subjectPanthera pardus fuscaen
dc.subjectAsian leopardsen
dc.subjectP. p. saxicoloren
dc.subjectP. p. fuscaen
dc.titleAssessing subspecies status of leopards (Panthera pardus) of northern Pakistan using mitochondrial DNAen
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Pest Management and Conservationen
lu.contributor.unitBio-Protection Research Centreen
dc.identifier.doi10.7717/peerj.7243en
dc.subject.anzsrc060802 Animal Cell and Molecular Biologyen
dc.subject.anzsrc0608 Zoologyen
dc.subject.anzsrc0604 Geneticsen
dc.subject.anzsrc0502 Environmental Science and Managementen
dc.subject.anzsrc06 Biological Sciencesen
dc.subject.anzsrc11 Medical and Health Sciencesen
dc.relation.isPartOfPeerJen
pubs.notesArticle e7243en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/ECOL
pubs.organisational-group/LU/BPRC
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/PE20
pubs.organisational-group/LU/Research Management Office/QE18
pubs.publication-statusPublished onlineen
pubs.volume7en
dc.identifier.eissn2167-8359en
dc.rights.licenceAttributionen
dc.rights.licenceAttributionen
lu.identifier.orcid0000-0001-7413-4704
lu.identifier.orcid0000-0003-4090-0815


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