Show simple item record

dc.contributor.authorPathrose, Blessy
dc.contributor.authorOutram, Megan
dc.contributor.authorJones, Elizabeth E.
dc.contributor.authorJaspers, Marlene V.
dc.contributor.authorRidgway, Hayley J.
dc.date.accessioned2019-11-19T21:04:05Z
dc.date.created2014-11-18en
dc.identifier.urihttps://hdl.handle.net/10182/11091
dc.description.abstractLaccases are a family of enzymes (polyphenol oxidases; PP0-1 and PP0-2) implicated in pathogenesis and degradation of lignin by many phytopathogens, including those that infect grapevines. The aim of this study was to (i) confirm that Ilyonectria species pathogenic to grapevines secrete laccase, (ii) to determine whether isolates vary in laccase secretion and (iii) to determine whether the amino acid sequence of laccase (/eel) differs between species. Laccase activity was measured using ABTS (2, 2¹-azino-bis [3-ethyl-benzthiazoline-6-sulfonic acid]) and DMP (2,6-dimethoxy-phenol). Six isolates of I. liriodendri and five isolates of the I. macrodidyma complex, including, I. macrodidyma (n=3), I. torrensensis and I. novozelandica were inoculated as agar plugs into minimal liquid media and incubated at 20°C for 7 days. The mycelium free extracellular fluid was assayed for PP0-1 and PP0-2 activity by their oxidation of ABTS and DMP, respectively. The results showed that all isolates produced PP0-1 activity but only seven produced detectable PP0-2 activity. There was isolate variation in both PP0-1 and PP0-2 activity for all species for which >1 isolate was tested (P<0.000). Degenerate PCR was used to amplify the feel gene from I. macrodidyma, I. novozelandica, I. torrensensis and I. liriodendri. Six amino acid polymorphisms were identified within isolates of I. liriodendri and the I. macrodidyma complex. Amino acid polymorphism was not found between isolates of the same species. Thus, variable laccase activity is likely to result from variable amount of enzyme secretion rather than isolate differences in enzyme activity.en
dc.language.isoen
dc.source9th International Workshop on Grapevine Trunk Diseasesen
dc.subjectlaccaseen
dc.titleVariable levels of laccase are secreted by four species of Ilyonectria that infect grapevinesen
dc.typeConference Contribution - unpublished
lu.contributor.unitLincoln University
lu.contributor.unitFaculty of Agriculture and Life Sciences
lu.contributor.unitDepartment of Pest Management and Conservation
pubs.finish-date2014-11-20en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/ECOL
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/PE20
pubs.organisational-group/LU/Research Management Office/QE18
pubs.publication-statusUnpublisheden
pubs.start-date2014-11-18en
dc.publisher.placeNational Wine Centre of Australia, Adelaideen
lu.identifier.orcid0000-0002-1879-4537
lu.subtypeConference Oral Presentationen


Files in this item

Default Thumbnail

This item appears in the following Collection(s)

Show simple item record