Research Archive

Fungal inoculum properties : Extracellular enzyme expression and pentachlorophenol removal by New Zealand Trametes species in contaminated field soils

Research Archive

Fungal inoculum properties : Extracellular enzyme expression and pentachlorophenol removal by New Zealand Trametes species in contaminated field soils

Ford, C. ; Walter, M. ; Northcott, G. ; Di, Hong J. ; Cameron, Keith C. ; Trower, T.
Type: Journal Article
Permalink: https://hdl.handle.net/10182/1131
Originally published: 2007 by American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America and available at http://jeq.scijournals.org/content/vol36/issue6/
Rights: © 2007 American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America
Keywords: pentachlorophenol; PCP; removal; bioremediation; soils; Trametes; white-rot fungi; Agronomy & Agriculture; Extracellular Matrix; Polyporales; beta-Galactosidase; Peroxidases; Soil Pollutants; Time Factors; New Zealand
Fields of Research: 05 Environmental Sciences; 06 Biological Sciences; 04 Earth Sciences

Abstract:

This study was conducted to improve the ability of indigenous New Zealand white-rot fungi to remove pentachlorophenol (PCP) from contaminated field soil. The effects of different bioaugmentation conditions on PCP removal and extracellular enzyme expression were measured in the laboratory. The conditions were fungal growth substrate and co-substrate composition, culture age, and Tween 80 addition to the contaminated soil. The fungi used were Trametes versicolor isolate HR131 and Trametes sp. isolate HR577. Maximum PCP removal was 70% after 7 wk from a 1043 mg kg⁻¹ PCP-contaminated soil inoculated with an 11-d-old fungal culture of T. versicolor isolate HR131. There was minimal production of undesirable pentachloroanisole by the fungi. Tween 80 addition had no affect on PCP removal. Poplar sawdust was more suitable as a fungal growth substrate and a co-substrate amendment for PCP removal and extracellular enzyme expression than the locally available pine and fir sawdust. Pentachlorophenol removal was not necessarily correlated with extracellular enzyme expression. The research results demonstrate that PCP biodegradation was affected by inoculum culture age, by the presence of a co-substrate amendment, and by growth substrate composition after white-rot fungal bioaugmentation into PCP-contaminated field soils.

Show full item record

Files in this item

[PDF]
Name: Fungal_Inoculum_P ...
Size: 771.8Kb
Format: PDF

This item appears in the following Collection(s)

Search Research Archive


Browse

My Account

Statistics