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dc.contributor.authorPurushotham, N.en
dc.contributor.authorJones, Elizabeth E.en
dc.contributor.authorMonk, J.en
dc.contributor.authorRidgway, Hayley J.en
dc.date.accessioned2020-02-24T19:23:18Z
dc.date.available2020-01-27en
dc.date.issued2020-01-27en
dc.date.submitted2020-01-23en
dc.identifier.urihttps://hdl.handle.net/10182/11453
dc.description.abstractAlthough the importance of the plant microbiome in commercial plant health has been well established, there are limited studies in native medicinal plants. Pseudowintera colorata (horopito) is a native New Zealand medicinal plant recognized for its antimicrobial properties. Denaturing gradient gel electrophoresis (DGGE) and Illumina MiSeq analysis of P. colorata plants from ten sites across New Zealand showed that tissue type strongly influenced the diversity and richness of endophytic bacteria (PERMANOVA, P < 0.05). In addition, two OTUs belonging to the genus Pseudomonas (Greengenes ID: 646549 and 138914) were found to be present in >75% of all P. colorata leaf, stem and root samples and were identified as the members of the P. colorata “core endomicrobiome”. Culture-independent analysis was complemented by the recovery of 405 endophytic bacteria from the tissues of P. colorata. Some of these cultured endophytic bacteria (n = 10) showed high antagonism against four different phytopathogenic fungi tested. The influence of endophytic bacteria on plant growth was assessed by inoculating P. colorata seedlings. The mean shoot height of seedlings treated with Bacillus sp. TP1LA1B were longer (1.83×), had higher shoot dry weight (1.8×) and produced more internodes (1.8×) compared to the control.en
dc.format.extent14en
dc.language.isoenen
dc.publisherMDPIen
dc.relationThe original publication is available from - MDPI - https://doi.org/10.3390/plants9020156en
dc.relation.urihttps://doi.org/10.3390/plants9020156en
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland.en
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectendophytic bacteriaen
dc.subjectendophytesen
dc.subjectplant-microbe interactionsen
dc.subjectplant growth promotionen
dc.subjectmicrobial ecologyen
dc.subjectmedicinal planten
dc.subjectIllumina MiSeqen
dc.titleCommunity structure, diversity and potential of endophytic bacteria in the primitive New Zealand medicinal plant Pseudowintera colorataen
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Pest Management and Conservationen
lu.contributor.uniten
lu.contributor.uniten
dc.identifier.doi10.3390/plants9020156en
dc.subject.anzsrc0607 Plant Biologyen
dc.subject.anzsrc0605 Microbiologyen
dc.subject.anzsrc060501 Bacteriologyen
dc.relation.isPartOfPlantsen
pubs.issue2en
pubs.notesArticle number 156en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/ECOL
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/2018 PBRF Staff group
pubs.publication-statusPublisheden
pubs.volume9en
dc.identifier.eissn2223-7747en
dc.rights.licenceAttributionen
dc.rights.licenceAttributionen
lu.identifier.orcid0000-0002-1879-4537


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