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dc.contributor.authorMao, Longfei
dc.contributor.authorVerwoerd, Wynand S.
dc.date.accessioned2020-02-25T23:35:56Z
dc.date.available2014-05-27en
dc.date.issued2014-11
dc.date.submitted2014-04-28en
dc.identifier.issn1389-1723en
dc.identifier.urihttps://hdl.handle.net/10182/11472
dc.description.abstractChlamydomonas reinhardtii possesses many potential advantages to be exploited as a biocatalyst in microbial fuel cells (MFCs) for electricity generation. In the present study, we performed computational studies based on flux balance analysis (FBA) to probe the maximum potential of C.reinhardtii for current output and identify the metabolic mechanisms supporting a high current generation in three different cultivation conditions, i.e., heterotrophic, photoautotrophic and mixotrophic growth. The results showed that flux balance limitations allow the highest current output for C.reinhardtii in the mixotrophic growth mode (2.368A/gDW), followed by heterotrophic growth (1.141A/gDW) and photoautotrophic growth the lowest (0.7035A/gDW). The significantly higher mediated electron transfer (MET) rate in the mixotrophic mode is in complete contrast to previous findings for a photosynthetic cyanobacterium, and was attributed to the fact that for C.reinhardtii the photophosphorylation improved the efficiency of converting the acetate into biomass and NADH production. Overall, the cytosolic NADH-dependent current production was mainly associated with five reactions in both mixotrophic and photoautotrophic nutritional modes, whereas four reactions participated in the heterotrophic mode. The mixotrophic and photoautotrophic metabolisms were alike and shared the same set of reactions for maximizing current production, whereas in the heterotrophic mode, the current production was additionally contributed by the metabolic activities in the two organelles: glyoxysome and chloroplast. In conclusion, C.reinhardtii has a potential to be exploited in MFCs of MET mode to produce a high current output.en
dc.format.extent565-574en
dc.language.isoen
dc.publisherElsevier on behalf of the Society for Biotechnology, Japan
dc.relationThe original publication is available from - Elsevier on behalf of the Society for Biotechnology, Japan - https://doi.org/10.1016/j.jbiosc.2014.04.021en
dc.relation.urihttp://www.sciencedirect.com/science/article/pii/S1389172314001522en
dc.relation.urihttps://doi.org/10.1016/j.jbiosc.2014.04.021en
dc.rightsCopyright © 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
dc.subjectmicrobial fuel cellen
dc.subjectChlamydomonas reinhardtiien
dc.subjectbioelectricityen
dc.subjectflux balance analysisen
dc.subjectflux variability analysisen
dc.subjectflux minimizationen
dc.subjectflux variability analysis with target flux minimizationen
dc.subjectBiotechnologyen
dc.titleComputational comparison of mediated current generation capacity of Chlamydomonas reinhardtii in photosynthetic and respiratory growth modesen
dc.typeJournal Article
lu.contributor.unitLincoln University
lu.contributor.unitFaculty of Agriculture and Life Sciences
lu.contributor.unitDepartment of Wine, Food and Molecular Biosciences
dc.identifier.doi10.1016/j.jbiosc.2014.04.021en
dc.relation.isPartOfJournal of Bioscience and Bioengineeringen
pubs.issue5en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/WFMB
pubs.publication-statusPublisheden
pubs.volume118en
dc.identifier.eissn1347-4421en


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