Biochemical responses associated with induced resistance to Colletotrichum acutatum in Pinus radiata seedlings treated with methyl jasmonate and Trichoderma spp.

Abstract

The effect of Trichoderma (T. atrobrunneum FCC320 and T. atroviride LU633) and/or methyl jasmonate (MJ) on resistance to terminal crook (Colletotrichum acutatum) and on seedling biochemistry was investigated in radiata pine (Pinus radiata) seedlings. Seedlings were germinated and grown in Trichoderma-amended or non-amended media for 3 months and then sprayed with 2.25 mM MJ 1 week before inoculation with C. acutatum. The incidence and severity of terminal crook in the seedlings treated with MJ and Trichoderma+MJ were lower than in Trichoderma-treated and Trichoderma untreated seedlings. The MJ-induced resistance response was concomitant with an increase in the concentrations of the monoterpenes α-pinene, β-pinene, β-phellandrene, camphene and myrcene in needles, and also α-pinene, β-pinene and camphene in stems. The concentrations of α-pinene, β-pinene and camphene were elevated from at least 1 week until 4 weeks after MJ application, compared with those in non-MJ counterparts. Trichoderma alone did not affect monoterpenes, but the concentrations of α-pinene, β-pinene and camphene were greater in needles of Trichoderma+MJ than in MJ-treated seedlings after 28 days. Total phenolic concentration in needles and peroxidase activity in stems were twofold greater in MJ-treated seedlings than in non-MJ seedlings over the same period. None of the treatments affected the activity of peroxidase in needles. It is proposed that the accumulation of monoterpene and phenolics and the induction of peroxidases contribute, in part, to MJ-induced resistance to terminal crook in radiata pine seedlings.