Oxidative damage in forage rape (Brassica napus L.) seeds following heat stress during seed development

Abstract

A short period (240°C hr; Tb = 25°C) of heat stress (30°C day/25°C night) during forage rape (Brassica napus L.) seed development or at seed physiological maturity can reduce seed vigour, but the extent of oxidative damage associated with this short heat stress was not known. Heat-stressed seeds were assessed for malondialdehyde (MDA) content, hydrogen peroxide (H₂O₂) accumulation, antioxidant enzyme activity, adenylate energy charge and seed ultrastructural integrity, and compared with that of non-heat-stressed seeds. Heat stress increased both MDA content and H₂O₂ accumulation by 35%–50%, reduced antioxidant enzyme activity by between 12% and 67%, and significantly reduced adenosine energy charge. Transmission electron microscope images showed clear evidence of seed deterioration in heat-stressed seeds, including ruptures in cell wall and plasma membranes, fused lipid bodies and damaged mitochondria. Heat stress at physiological maturity caused more oxidative damage than the same heat stress during seed development. Seed vigour decreased as H₂O₂ accumulation increased and antioxidant enzyme activity decreased, but no direct relationship between lipid peroxidation and seed vigour was established. The extent of damage resulting from even shorter periods of heat stress (<240°C hr) before or at seed physiological maturity requires investigation.