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dc.contributor.authorGong, Hua
dc.contributor.authorZhou, Huitong
dc.contributor.authorWang, Jiqing
dc.contributor.authorLi, Shaobin
dc.contributor.authorLuo, Yuzhu
dc.contributor.authorHickford, Jonathan G. H.
dc.date.accessioned2020-05-25T00:46:58Z
dc.date.available2019-10-26en
dc.date.issued2019-11-01
dc.date.submitted2019-10-23en
dc.identifier.issn2073-4425en
dc.identifier.urihttps://hdl.handle.net/10182/11920
dc.description.abstractThe keratin-associated proteins (KAPs) are structural components of hair/wool fibres. All of the KAPs identified to date contain cysteine, which is thought to form disulphide bonds cross-linking the keratin intermediate filaments. Here, we report the identification of a KAP gene in sheep that would produce a protein that contains a high proportion (63.2 mol%) of glycine and tyrosine, but would not contain any cysteine. This suggests that other forms of intra- and inter-strand interaction may occur with this KAP, such as interactions via ring-stacking and hydrogen-bonding. The gene was dissimilar to any previously reported KAP gene, and was therefore assigned to a new family, and named KRTAP36-1. The KRTAP36-1 genome sequence was almost identical to some EST sequences from sheep and goat skin follicles, suggesting that it is present and expressed in sheep and goats. A BLAST search of the human genome assembly sequence did not reveal any human homologue. Three variant sequences (named A to C) of ovine KRTAP36-1 were identified and four single nucleotide polymorphisms (SNPs) were detected. One SNP was located 32 bp upstream of the coding region, and all of the others were in the coding region and were nonsynonymous. After correcting for potential linkage to the proximal KRTAP20-1, variant B of KRTAP36-1 was found to be associated with increased prickle factor (PF) in wool, suggesting that variation in the gene may have the potential to be used as gene marker for breeding sheep with lower PF.en
dc.format.extent12en
dc.language.isoen
dc.publisherMDPI
dc.relationThe original publication is available from - MDPI - https://doi.org/10.3390/genes10110848en
dc.relation.urihttps://doi.org/10.3390/genes10110848en
dc.rights© 2019 by the authors
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectkeratin-associated protein KAP36-1 gene (KRTAP36-1)en
dc.subjectvariationen
dc.subjectwool traitsen
dc.subjectprickle factoren
dc.subjectsheepen
dc.subject.meshWoolen
dc.subject.meshAnimalsen
dc.subject.meshCysteineen
dc.subject.meshTyrosineen
dc.subject.meshGlycineen
dc.subject.meshAmino Acid Substitutionen
dc.subject.meshPolymorphism, Single Nucleotideen
dc.subject.meshHydrogen Bondingen
dc.subject.meshFemaleen
dc.subject.meshKeratinsen
dc.subject.meshProtein Domainsen
dc.subject.meshWool Fiberen
dc.titleCharacterisation of an ovine keratin associated protein (KAP) gene, which would produce a protein rich in glycine and tyrosine, but lacking in cysteineen
dc.typeJournal Article
lu.contributor.unitLincoln University
lu.contributor.unitFaculty of Agriculture and Life Sciences
lu.contributor.unitDepartment of Agricultural Sciences
dc.identifier.doi10.3390/genes10110848en
dc.subject.anzsrc0702 Animal Productionen
dc.subject.anzsrc060408 Genomicsen
dc.subject.anzsrc0601 Biochemistry and Cell Biologyen
dc.relation.isPartOfGenesen
pubs.issue11en
pubs.notesArticle ID 848en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/AGSC
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/QE18
pubs.publication-statusPublisheden
pubs.volume10en
dc.identifier.eissn2073-4425en
dc.rights.licenceAttributionen
dc.rights.licenceAttributionen
lu.identifier.orcid0000-0002-2247-3824
lu.identifier.orcid0000-0001-9313-761X
lu.identifier.orcid0000-0001-7620-3160


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