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dc.contributor.authorWang, Jiqingen
dc.contributor.authorZhou, Huitongen
dc.contributor.authorHickford, Jonathan G. H.en
dc.contributor.authorHao, Zhiyunen
dc.contributor.authorShen, Jiyuanen
dc.contributor.authorLuo, Yuzhuen
dc.contributor.authorHu, Jiangen
dc.contributor.authorLiu, Xiuen
dc.contributor.authorLi, Shaobinen
dc.date.accessioned2020-06-23T23:39:40Z
dc.date.available2020-05-21en
dc.date.issued2020-05-21en
dc.date.submitted2020-04-16en
dc.identifier.urihttps://hdl.handle.net/10182/12122
dc.description.abstractSmall-Tailed Han (STH) sheep are known for their high fecundity, but the survival of lambs is compromised and influences the commercial return from farming these sheep, with this being attributed in part to starvation from insufficient milk production by the ewes. In this study, the transcriptome profiles of the mammary gland of lactating and non-lactating STH ewes were investigated using paired-end RNA sequencing (RNA-Seq). An average of 14,447 genes were found to be expressed at peak-lactation in the STH sheep, while 15,146 genes were expressed in non-lactating ewes. A total of 4,003 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that the DEGs were associated with a wide range of cellular components, biological processes and metabolic pathways, including binding activities, signaling pathways, cellular structures, and immune responses. The most highly expressed genes at peak-lactation included CSN2, LGB, LALBA, CSN1S1, CSN1S2, and CSN3, and the 10 most highly expressed genes accounted for 61.37% of the total Reads Per Kilobase of transcript, per Million mapped reads (RPKM). The most highly expressed genes in the mammary gland of non-lactating ewes included IgG, THYMB4X, EEF1A1, IgA, and APOE, and the 10 most highly expressed genes accounted for only 12.97% of the total gene RPKM values. This suggests that the sheep mammary gland undergoes a substantial development in milk protein synthesis infrastructure and promotion of protein transportation during lactation.en
dc.format.extent11en
dc.language.isoenen
dc.publisherFrontiers Media S.A.en
dc.relationThe original publication is available from - Frontiers Media S.A. - https://doi.org/10.3389/fgene.2020.00472en
dc.relation.urihttps://doi.org/10.3389/fgene.2020.00472en
dc.rights© 2020 Wang, Zhou, Hickford, Hao, Shen, Luo, Hu, Liu and Li.en
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectRNA-Seqen
dc.subjectmammary glanden
dc.subjectdifferentially expressed gene (DEG)en
dc.subjectlactationen
dc.subjectnon-lactationen
dc.subjectSmall-Tailed Han sheepen
dc.subjectdifferentially expressed gene (DGE)en
dc.titleComparison of the transcriptome of the ovine mammary gland in lactating and non-lactating Small-Tailed Han sheepen
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Agricultural Sciencesen
dc.identifier.doi10.3389/fgene.2020.00472en
dc.subject.anzsrc0702 Animal Productionen
dc.subject.anzsrc060408 Genomicsen
dc.subject.anzsrc0601 Biochemistry and Cell Biologyen
dc.subject.anzsrc07 Agricultural and Veterinary Sciencesen
dc.subject.anzsrc0604 Geneticsen
dc.subject.anzsrc1103 Clinical Sciencesen
dc.subject.anzsrc1801 Lawen
dc.relation.isPartOfFrontiers in Geneticsen
pubs.notesArticle 472en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/AGSC
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/QE18
pubs.publication-statusPublisheden
pubs.volume11en
dc.identifier.eissn1664-8021en
dc.rights.licenceAttributionen
dc.rights.licenceAttributionen
lu.identifier.orcid0000-0001-9313-761X
lu.identifier.orcid0000-0001-7620-3160


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