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dc.contributor.authorFroehlich, Kelly A.en
dc.contributor.authorGreer, Andrew W.en
dc.contributor.authorBryant, Racheal H.en
dc.date.accessioned2020-08-25T21:22:57Z
dc.date.issued2019en
dc.identifier.issn0370-2731en
dc.identifier.urihttps://hdl.handle.net/10182/12563
dc.description.abstractLoline, an alkaloid produced by Neotyphodium endophytes in pastoral grass species with potential antimicrobial properties, has several known derivatives; N-formyl loline (NFL), N-acetyl loline (NAL), N-acetyl norloline (NANL), N-methyl loline (NML), and loline base, though the availability and form during rumen fermentation and digestion is unclear. Festuca pratensis seeds either with (LOL) or without (NIL) lolines (150-200 μg/mL) were incubated in either sterile or viable rumen fluid or in either HCL/pepsin (pH 2) or water (pH 7). At 72 hours LOL in sterile rumen fluid had 23%, 26%, and 45% more NFL (P=0.05), NANL (P=0.04), and loline base (P=0.01) respectively, with 27% more total loline present (P=0.06), and 65% more NML (P=0.09) compared with LOL in viable rumen fluid. Loline tended to alter the fermentation pattern as NIL produced 11% more ammonia (P=0.07), and 5% less propionate (P=0.06) than LOL. In HCL/pepsin there were 38%, 49%, and 39% more total lolines (P=0.05), NANL (P=0.02), and NFL (P=0.04) respectively, compared with water. Rumen and abomasal digestion appear to have a small effect on loline’s form and concentration with only slight alteration to the rumen fermentation pattern. Presence of lolines had little evidence of an antimicrobial effect.en
dc.format.extent13-19en
dc.language.isoenen
dc.publisherNew Zealand Society of Animal Productionen
dc.relationThe original publication is available from - New Zealand Society of Animal Production - http://www.nzsap.org/proceedings/effect-vitro-ruminal-fermentation-and-hclpepsin-digestion-lolineen
dc.rights© The Authorsen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectlolineen
dc.subjectalkaloiden
dc.subjectruminal digestionen
dc.subjectabomasal digestionen
dc.titleEffect of in-vitro ruminal fermentation and HCL/pepsin digestion on lolineen
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Agricultural Sciencesen
dc.subject.anzsrc0702 Animal Productionen
dc.subject.anzsrc070204 Animal Nutritionen
dc.relation.isPartOfNew Zealand Journal of Animal Science and Productionen
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/AGSC
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/PE20
pubs.organisational-group/LU/Research Management Office/QE18
pubs.publication-statusPublisheden
pubs.publisher-urlhttp://www.nzsap.org/proceedings/effect-vitro-ruminal-fermentation-and-hclpepsin-digestion-lolineen
pubs.volume79en
dc.rights.licenceAttribution-NonCommercial-NoDerivativesen
lu.identifier.orcid0000-0002-1479-3297
lu.identifier.orcid0000-0002-5004-8147


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