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    Determination of bitter acids in South Island grown hops by UPLC : A Dissertation submitted in partial fulfilment of the requirements for the Degree of Master of Science in Food Innovation at Lincoln University

    Wu, Bin
    Abstract
    In this study, ultra-performance liquid chromatography was used to quantify the content of alpha acids in hops grown in Nelson, Canterbury and Southland. This method used a binary solvent system consisting of (1) 100% methanol (A) and (2) 85% of solvent A and 15% of solvent B. B is 0.17% v/v formic acid and water. Analysis was performed isocratically at a flowrate of 0.2 mL/min. Separation was achieved on the column of Acquity UPLC® BEH C18, 50 x 2.1 mm, with a particle size of 1.7μm, and with a Acquity UPLC® BEH C18 1.7μm, VanGuard™ pre-column 5 x 2.1 mm. The compounds of interest eluted within 4 mins. The results of this experimental study show that the content of alpha acids in hops is not only related to the growth environment but also related to the variety.... [Show full abstract]
    Keywords
    Ultra Performance Liquid Chromatography (UPLC); Humulus lupulus L.; alpha acids; beta acids
    Fields of Research
    0908 Food Sciences; 090899 Food Sciences not elsewhere classified; 090801 Food Chemistry and Molecular Gastronomy (excl. Wine)
    Date
    2021
    Type
    Dissertation
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    • Department of Wine, Food and Molecular Biosciences [713]
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