Variation in PLIN2 and its association with milk traits and milk fat composition in dairy cows
Variation in PLIN2 and its association with milk traits and milk fat composition in dairy cows
Li, YH ; Zhou, Huitong ; Cheng, L ; Zhao, J ; Hickford, Jonathan
Li, YH
Zhou, Huitong
Cheng, L
Zhao, J
Hickford, Jonathan
Date
2021-03-30
Type
Journal Article
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ANZSRC::0702 Animal Production , ANZSRC::060499 Genetics not elsewhere classified , ANZSRC::100104 Genetically Modified Animals , ANZSRC::30 Agricultural, veterinary and food sciences
Abstract
The current study investigated associations between variation in the bovine perilipin-2 gene (PLIN2) and milk traits (milk fat content, milk protein content, milk yield and milk fatty acid (FA) component levels) in 409 New Zealand pasture-grazed Holstein-Friesian × Jersey-cross (HF × J-cross or Kiwicross™) cows. Five nucleotide sequence variants were found in three regions of the gene, including c.17C>T in exon 2, c.53A>G in exon 3, c.595+23G>A and c.595+104_595+108del in intron 5, and c.∗302T>C in the 3′-untranslated region. The c.∗302T>C substitution produces two nucleotide sequence variants (A₅ and B₅), and this variation was associated with variation in milk protein content and milkfat composition for C10:0, C11:0, C12:0, C13:0 and C16:0 FA and medium-chain fatty acid (MCFA) and long-chain fatty acid (LCFA) groups. After correcting for the effect of variation in the diacylglycerol acyl-CoA acyltransferase 1 gene (DGAT1) that results in the amino acid substitution p.K232A, variation in the FA binding protein 4 gene (FABP4) and variation in the stearoyl-CoA desaturase ( "-9-desaturase) gene (SCD) that results in the amino acids substitution p.A293V, significant differences between A₅A₅ and B₅B₅ cows were found for C10:0, C11:0, C12:0, C13:0, C16:0, and the MCFA, LCFA, total saturated FA and C10:1 index groups. This suggests that nucleotide sequence variation in PLIN2 may be affecting milk FA component levels.
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© The Author(s), 2021.