Show simple item record

dc.contributor.authorWilliams, Christen Lenney
dc.date.accessioned2010-03-23T22:50:36Z
dc.date.available2010-03-23T22:50:36Z
dc.date.issued1994
dc.identifier.urihttps://hdl.handle.net/10182/1545
dc.description.abstractThe Argentine stem weevil, Listronotus bonariensis (Kuschel) (Coleoptera: Curculionidae), is an important introduced pasture pest in New Zealand and causes hundreds of millions of dollars in damage annually to pasture and livestock health. Adult L. bonariensis were collected from 9 diverse locations in South America (the species' native habitat), 5 locations in New Zealand and 1 location in Australia (where the species is also an introduced pest). The 15 populations were studied by 2 DNA-based techniques, RAPD and mitochondrial analysis, in an effort to determine the geographical origin of the pest populations in New Zealand. RAPD revealed similarities between populations sampled within New Zealand, as well as polymorphisms between the South American populations. Mitochondrial restriction fragment length analysis revealed the unusually large mitochondrial genome size of approximately 32 kilobases in this species but negligible variation within or between populations. Based on the RAPD results, the Rio de la Plata region on the east coast of South America is thought to be the geographical source for the Listronotus bonariensis (Kuschel) populations in New Zealand. The discrepancy between the levels of genetic variation revealed by RAPD and mitochondrial analysis in the native populations is discussed and hypotheses are presented to reconcile this difference.en
dc.language.isoenen
dc.publisherLincoln Universityen
dc.rights.urihttps://researcharchive.lincoln.ac.nz/page/rights
dc.subjectgenetic diversityen
dc.subjectintroduced speciesen
dc.subjectcolonized populationsen
dc.subjectRandom Amplified Polymorphic DNA (RAPD)en
dc.subjectmitochondrial DNA (mtDNA)en
dc.subjectmitochondrial restriction fragment length polymorphisms (mtRFLP)en
dc.subjectListronotus bonariensisen
dc.subjectKuschelen
dc.subjectweevilsen
dc.subjectpestsen
dc.titleGenetic diversity in Listronotus bonariensis (Kuschel) and geographical origin of the New Zealand populationen
dc.typeThesisen
thesis.degree.grantorLincoln Universityen
thesis.degree.levelDoctoralen
thesis.degree.nameDoctor of Philosophyen
dc.subject.marsdenFields of Research::300000 Agricultural, Veterinary and Environmental Sciences::300200 Crop and Pasture Production::300204 Plant protection (pests, diseases and weeds)en
dc.subject.marsdenFields of Research::270000 Biological Sciences::270400 Botany::270403 Plant pathologyen
lu.thesis.supervisorBullock, David
lu.contributor.unitDepartment of Agricultural Sciencesen
dc.rights.accessRightsDigital thesis can be viewed by current staff and students of Lincoln University only. If you are the author of this item, please contact us if you wish to discuss making the full text publicly available.en


Files in this item

Default Thumbnail

This item appears in the following Collection(s)

Show simple item record