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dc.contributor.authorChua, C. G.
dc.date.accessioned2010-04-27T21:47:12Z
dc.date.available2010-04-27T21:47:12Z
dc.date.issued1975
dc.identifier.urihttps://hdl.handle.net/10182/1760
dc.description.abstract1. Rat (Wistar, Rattus norvegicus) was found to possess six distinctive haemoglobin components. The major haemoglobin component E was prepared by chromatography on column of DEAE-Sephadex using a pH gradient. Globin chains were separated on a CM-cellulose column which was eluted with increasing ionic strength of sodium ions in the presence of 8 M urea. 2. The amino acid sequence of the ∝ chain was substantially determined. The soluble tryptic peptides prepared from the native ∝ globin and from the aminoethylated ∝ globin were separated by peptide mapping. Sequencing of the tryptic peptides was carried out by the dansyl-Edman method and by the overlapping of smaller peptide fragments derived from secondary enzymic digestion. The insoluble core peptides were further digested with chymotrypsin, thermolysin and pepsin to give smaller soluble peptides for sequencing. The tryptic peptides were ordered on the basis of their homology with the corresponding peptides of human ∝ chain. 3. The amino acid sequence obtained in this study was compared with an amino acid sequence which was partially determined by an automated sequencer and partially by the homology method (Garrick et al. 1975). Disagreements between the two sequences were discussed and the favoured amino acid sequence for the rat major ∝ chain was proposed. 4. Residue 13(All) ∝ of rat is a cysteine, which is unique among the mammalian ∝ globins. The occurrence of this externally situated cysteine was suggested to be responsible for the in vitro crystallizing property of rat haemoglobins. 5. Structural and functional constraints of the haemoglobin molecule were shown to be a major selective force in the evolution of the rat ∝ chain. 6. A phylogenetic tree of the ∝ globins of rat, mouse, dog and rabbit was built by reconstructing their ancestral sequences. From this the time of divergence of rat (Rattus) and mouse (Mus) was calculated to be approximately 52-58 million years ago.en
dc.language.isoenen
dc.publisherLincoln College, University of Canterburyen
dc.rights.urihttps://researcharchive.lincoln.ac.nz/page/rights
dc.subjectratsen
dc.subjectRattus norvegicusen
dc.subjectWistar raten
dc.subjectamino acid sequenceen
dc.subjectalpha globulinen
dc.subjecthaemoglobin componentsen
dc.subjectcysteineen
dc.subjecttryptic peptideen
dc.titleThe amino acid sequence of the major alpha globin of the rat (Wistar, Rattus norvegicus)en
dc.typeThesisen
thesis.degree.grantorUniversity of Canterburyen
thesis.degree.levelDoctoralen
thesis.degree.nameDoctor of Philosophyen
dc.subject.marsdenFields of Research::270000 Biological Sciences::270100 Biochemistry and Cell Biology::270101 Analytical biochemistryen
dc.subject.marsdenFields of Research::270000 Biological Sciences::270100 Biochemistry and Cell Biology::270109 Structural biologyen
lu.thesis.supervisorCarrell, R. W.
lu.contributor.unitDepartment of Ecologyen
dc.rights.accessRightsDigital thesis can be viewed by current staff and students of Lincoln University only. Print copy available for reading in Lincoln University Library. May be available through inter-library loan.en


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