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    Genetic polymorphism of the human apolipoproteins A and E

    Joyce, Steve
    Abstract
    The technique of horizontal, thin layer isoelectric focusing was used to demonstrate the polymorphism of the apolipoproteins E, A-I, A-II and A-IV. Polymorphic forms of apo E were detected after ultracentrifugation of plasma, delipidation of VLDL and isoelectric focusing of the remaining protein. The six common phenotypes, homozygotes E 2/2, E 3/3, E 4/4 and the heterozygotes E 2/3, E 2/4, E 3/4, of apo E were demonstrated. A simplified one step screening method, not requiring ultracentrifugation techniques, was used to demonstrate the A apolipoproteins. As the method uses native serum, epo A-IV was detected as well as apo A-I and apo A-II. The one step screening technique demonstrated reduced levels of apo A-I and A-II in a patient homozygous for Tangier disease. It was also utilised to screen patients undergoing cardiac catheterisation, no variants of apo A-I and A-II were detected in this patient population. The presence of a minor apo A-IV variant in these patients requires confirmation by other techniques.... [Show full abstract]
    Keywords
    genetic polymorphism; apolipoproteins; Tangier disease; lipid disorders; plasma lipoproteins; lipoprotein cholesterol; genetic risk factors; apolipoprotein A; apolipoprotein E; very low density lipoprotein (VLDL)
    Fields of Research
    110104 Medical Biochemistry: Lipids; 110106 Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
    Date
    1985
    Type
    Thesis
    Access Rights
    Digital thesis can be viewed by current staff and students of Lincoln University only. Print copy available for reading in Lincoln University Library.
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    • Theses and Dissertations with Restricted Access [2207]
    • Department of Wine, Food and Molecular Biosciences [631]
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