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    Re-establishing plantlets from tissue culture: a review

    Conner, A. J.; Thomas, Michael B.
    Abstract
    The literature pertinent to the re-establishment of tissuecultured plants in vivo is reviewed. The difficulties associated with survival and growth of tissue cultured plants after transplantation are attributed to the poor control of water loss from the plants and their necessity to switch from heterotrophic to photoautotrophic nutrition. Aspects discussed include: the possibility of transplanting directly from stage II shoot proliferation cultures and rooting in vivo; the importance of stage III culturing for preconditioning plants prior to transplantation; the optimum sizes of propagules and substrate preferences for transplantation; stress reduction, disease prevention, and the importance of humidity, temperature and light levels during transplantation. The relative merits of numerous approaches to transplantation by various workers for many species are discussed. Suggestions of areas in which further work is urgently required are given, along with some recommendations and general guidelines for the re-establishment of tissue cultured plants in vivo.... [Show full abstract]
    Keywords
    tissue cultured plants; nursery propagation; rooting; nursery plants
    Fields of Research
    060702 Plant Cell and Molecular Biology; 060704 Plant Pathology; 060703 Plant Developmental and Reproductive Biology
    Date
    1982
    Type
    Journal Article
    Collections
    • Department of Agricultural Sciences [1445]
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    Citation
    Conner, A. J., & Thomas, M. B. (1982). Re-establishing plantlets from tissue culture: a review. In The International Plant Propagators' Society Combined Proceedings, 31, 1981. (pp. 342-357). The International Plant Propagators' Society.
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