Research@Lincoln
    • Login
     
    View Item 
    •   Research@Lincoln Home
    • Theses and Dissertations
    • Theses and Dissertations with Restricted Access
    • View Item
    •   Research@Lincoln Home
    • Theses and Dissertations
    • Theses and Dissertations with Restricted Access
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Selection of a ScFv against a sheep parasite antigen using phage-display

    Gourley, Tania
    Abstract
    Trichostrongylus colubriformis is an important parasite in sheep and causes millions of dollars damage annually. Current control methods rely almost exclusively on anthelmintic drugs to which strains of T. colubriformis are rapidly becoming resistant. A recombinant protein antigen, T-17, from the fourth larval stage of T. colubriformis was obtained. Single-chain Fv (ScFv) antibody libraries were made from two hybridoma cell-lines each expressing a single anti-T-17 antibody. The two ScFv libraries were displayed on filamentous bacteriophage then panned against the T-17 antigen but failed to produce a functional anti-T-17 ScFv. A more diverse ScFv library was then made from the spleen of a mouse immunised with the T-17 antigen. Several phage-display panning methods were used and compared, one method using a two-step acid elution of the bound phage succeeded in selecting a clone producing ScFvs binding to the T -17 antigen which runs at 19 kDa under reducing conditions. The ScFvs from this clone and all of the other clones which gave positive T-17-binding signals in colony lift-assays and ELISA were binding to proteins in the T -17 preparation which ran at 37.5, 31, and 16.5 kDa. Evidence suggesting that these three proteins are also T-17 is discussed.... [Show full abstract]
    Keywords
    ScFv; filamentous phage-display; E. coli antibody expression; sheep parasites; Trichostrongylus colubriformis; sheep diseases
    Date
    1996
    Type
    Thesis
    Access Rights
    Digital thesis can be viewed by current staff and students of Lincoln University only. If you are the author of this item, please contact us if you wish to discuss making the full text publicly available.
    Collections
    • Theses and Dissertations with Restricted Access [2370]
    • Department of Agricultural Sciences [1569]
    View/Open
    Staff/student login to read
    Share this

    on Twitter on Facebook on LinkedIn on Reddit on Tumblr by Email

    Metadata
     Expand record
    This service is maintained by Learning, Teaching and Library
    • Archive Policy
    • Copyright and Reuse
    • Deposit Guidelines and FAQ
    • Contact Us
     

     

    Browse

    All of Research@LincolnCommunities & CollectionsTitlesAuthorsKeywordsBy Issue DateThis CollectionTitlesAuthorsKeywordsBy Issue Date

    My Account

    LoginRegister

    Statistics

    View Usage Statistics
    This service is maintained by Learning, Teaching and Library
    • Archive Policy
    • Copyright and Reuse
    • Deposit Guidelines and FAQ
    • Contact Us