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dc.contributor.authorMcCormick, Daniel
dc.date.accessioned2011-06-27T00:21:35Z
dc.date.available2011-06-27T00:21:35Z
dc.date.issued1995
dc.identifier.urihttps://hdl.handle.net/10182/3660
dc.description.abstractSodium monofluoroacetate (1080) is a highly effective vertebrate pesticide that is widely used in New Zealand for control of mammalian pests. 1080 toxicity results from its conversion in the mitochondria to fluorocitrate, a competitive inhibitor and an irreversible in activator of aconitase. Blockage of TCA cycle, depletion of cellular energy, and organ failure are the fatal consequences of the inhibition of mitochondrial aconitase. However, being a non-selective toxin it poses high risk to the lives of non-target species as a result of accidental poisoning. Our ultimate goal is to develop an effective antidote that reverses the inhibition of mitochondrial aconitase caused by 1080. This study summarizes the factors I considered while standardizing an assay for measuring aconitase activity including methods devised in overcoming the artefacts, experiments carried out for testing the validity of the established assay procedure, and the stability of the enzyme preparations. I applied techniques that allowed the measurement of aconitase in both mitochondrial and cytoplasmic fractions of rat liver, intactness of isolated mitochondria, and the release of matrix contents by the disruption of mitochondria. Systematic studies using 1080 and fluorocitrate revealed that 1080 has no direct effect on aconitase, and inhibition of aconitase is possible only after the conversion of fluoroacetate to fluorocitrate. I standardized the conditions that effectively bring about the conversion in vitro by using isolated mitochondria. The knowledge and information obtained through this work can now be used in further in vitro and in vivo studies on the development of antidotes. The methods will be particularly useful in screening for compounds with antidotal properties.en
dc.language.isoenen
dc.publisherLincoln Universityen
dc.rights.urihttps://researcharchive.lincoln.ac.nz/page/rights
dc.subjectaconitaseen
dc.subjectsodium monofluoroacetateen
dc.subject1080en
dc.subjectfluorocitrateen
dc.subjectinhibitionen
dc.subjectmitochondriaen
dc.subjectcytoplasmen
dc.subjectin vitro activityen
dc.subjectbackground activityen
dc.subject1080 toxicityen
dc.title1080 toxicity - in vitro aconitase studiesen
dc.typeDissertationen
thesis.degree.grantorLincoln Universityen
thesis.degree.levelOtheren
thesis.degree.nameBachelor of Scienceen
lu.thesis.supervisorGooneratne, Ravi
lu.thesis.supervisorKantham, Lakshmi
lu.thesis.supervisorMason, Sue
lu.contributor.unitDepartment of Wine, Food and Molecular Biosciencesen
dc.rights.accessRightsThis digital dissertation can be viewed by current staff and students of Lincoln University only.en
dc.subject.anzsrc0601 Biochemistry and Cell Biologyen


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