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dc.contributor.authorCouldrey, Christine
dc.date.accessioned2011-08-01T04:55:16Z
dc.date.available2011-08-01T04:55:16Z
dc.date.issued1994
dc.identifier.urihttps://hdl.handle.net/10182/3747
dc.description.abstractThere is evidence that variation in the calpastatin gene exists among breeds of cattle. It has been suggested that such variation contributes to differences in muscle growth and postmortem conditioning of meat. The objective of this work was to determine if variation also exists in the calpastatin gene of sheep. Digestion of sheep DNA with BamHI restriction endonuclease, gave identifiable bands when probed with a bovine calpastatin cDNA probe. Reproducible variation, similar to that reported for cattle genomic DNA was detected in the banding pattern among breeds of sheep. Further variation in the ovine calpastatin gene was identified using primers designed to the bovine calpastatin gene and optimised PCR-SSCP conditions. In the breeds examined, two alleles, inherited in a Mendelian fashion were discovered by examination of small families. Either of the techniques used to detect variation/polymorphism in the calpastatin gene in these studies, may be further examined for its linkage to meat tenderness. Ultimately this may lead to the selection for reduced calpastatin activity to enhance meat quality by increasing meat tenderness.en
dc.language.isoenen
dc.publisherLincoln Universityen
dc.rights.urihttps://researcharchive.lincoln.ac.nz/page/rights
dc.subjectcalpastatinen
dc.subjectovineen
dc.subjectmeat tendernessen
dc.subjectRFLPen
dc.subjectSSCPen
dc.titlePolymorphism in the ovine calpastatin geneen
dc.typeDissertationen
thesis.degree.grantorLincoln Universityen
thesis.degree.levelOtheren
thesis.degree.nameBachelor of Scienceen
lu.thesis.supervisorBickerstaffe, Roy
lu.thesis.supervisorHickford, Jon
lu.contributor.unitDepartment of Agricultural Sciencesen
dc.rights.accessRightsDigital dissertation can be viewed by current staff and students of Lincoln University only.en
dc.subject.anzsrc0601 Biochemistry and Cell Biologyen
dc.subject.anzsrc060601 Animal Physiology - Biophysicsen


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