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dc.contributor.authorJones, Elizabeth E.en
dc.contributor.authorCarpenter, Margaret A.en
dc.contributor.authorFong, D.en
dc.contributor.authorGoldstein, A. L.en
dc.contributor.authorThrush, A.en
dc.contributor.authorCrowhurst, R. N.en
dc.contributor.authorStewart, Alisonen
dc.date.accessioned2008-03-26T00:34:16Z
dc.date.issued1999-08en
dc.identifier.citationJones, E., Carpenter, M., Fong, D., Goldstein, A., Thrush, A., Crowhurst, R., & Stewart, A. (1999). Co-transformation of the sclerotial mycoparasite Coniothyrium minitans with hygromycin B resistance and β-glucuronidase markers. Mycological Research, 103(8), 929-937.en
dc.identifier.issn0953-7562en
dc.identifier.urihttps://hdl.handle.net/10182/375
dc.description.abstractConiothyrium minitans was successfully co-transformed with the uidA (β-glucuronidase) and the hygromycin-resistance (hph) genes. Both were under the control of the glyceraldehyde-3-phosphate promoter from Aspergillus nidulans. Hygromycin resistance was used as a selectable marker for transformation. In successive transformation experiments, transformation frequencies of up to 1000 transformants µg⁻¹ of plasmid DNA were obtained for isolate A69. Of the ten monospore hygromycin-resistant cultures tested, nine also expressed the uidA gene. Expression of hph and uidA was stable in all transformants after several months of successive subculturing on non-selective medium, and after passage through a sclerotium of Sclerotinia sclerotiorum. Southern hybridization analyses showed all transformants carried multiple copies of each marker gene. When grown on PDA, the culture morphology of three of the transformants of (T×2, T×3 and T×4) was similar to the wild type. Four of the five transformants (T×3, T×4, T×21 and T×24) grew as well as the wild type on different media, and responded to changes in water potential in a similar manner to the wild type. All five transformants were equally parasitic on sclerotia of S. sclerotiorum compared with the wild type. Transformants T×3 and T×4 were the most similar to the wild type in biological characteristics and will be used in future studies. The results indicate that hph- and uidA-transformed strains of C. minitans will be useful for ecological studies on its survival and dissemination.en
dc.format.extent929-937en
dc.language.isoenen
dc.publisherCambridge University Pressen
dc.relationThe original publication is available from - Cambridge University Press - http://hdl.handle.net/10182/375en
dc.rightsCopyright © The British Mycological Society 1999en
dc.subjectConiothyrium minitansen
dc.subjectmycoparasiteen
dc.subjectmarker genesen
dc.subjectMycology & Parasitologyen
dc.titleCo-transformation of the sclerotial mycoparasite Coniothyrium minitans with hygromycin B resistance and [beta]-glucuronidase markersen
dc.typeJournal Article
dc.subject.marsdenFields of Research::270000 Biological Sciences::270300 Microbiology::270305 Mycologyen
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitPest-Management and Conservationen
lu.contributor.unitBio-Protection and Ecologyen
lu.contributor.unitBio-Protection Research Centreen
lu.contributor.unitResearch Management Officeen
lu.contributor.unit/LU/Research Management Office/2018 PBRF Staff groupen
dc.subject.anzsrc0605 Microbiologyen
dc.subject.anzsrc0607 Plant Biologyen
dc.relation.isPartOfMycological Researchen
pubs.issue8en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/ECOL
pubs.organisational-group/LU/BPEC
pubs.organisational-group/LU/BPRC
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/2018 PBRF Staff group
pubs.publication-statusPublisheden
pubs.publisher-urlhttp://hdl.handle.net/10182/375en
pubs.volume103en
lu.identifier.orcid0000-0002-1879-4537


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