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dc.contributor.authorGately, Karlen
dc.date.accessioned2011-08-24T04:12:49Z
dc.date.issued1996en
dc.identifier.urihttps://hdl.handle.net/10182/3809
dc.description.abstractp94 is a muscle specific member of the calpain family that undergoes rapid autolysis in vivo, and has been linked to Limb-girdle muscular dystrophy type 2A (LMGD2A). To ascertain whether polymorphism within or near the p94 gene was responsible for different muscle phenotypes, an investigation of intron 6 was undertaken. A polymerase chain reaction (peR) involving Expand high fidelity (EHF) polymerase was developed for amplification of the region. The amplimer was investigated by polyacrylamide gel electrophoresis (PAGE) and PAGE analysis of EcoR1 digests. The results from the investigation suggest that there is no polymorphic variation between individuals within intron 6 of p94 but that there is a species difference between cattle and sheep.en
dc.language.isoenen
dc.publisherLincoln Universityen
dc.subjectp94en
dc.subjectpolymerase chain reaction (PCR)en
dc.subjectcalpainen
dc.subjectmeat tendernessen
dc.titleAmplification of the p94 (n-calpain) gene of sheep and cattleen
dc.typeThesis
thesis.degree.grantorLincoln Universityen
thesis.degree.levelOtheren
thesis.degree.nameBachelor of Scienceen
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
dc.rights.accessRightsDigital dissertation can be viewed by current staff and students of Lincoln University only.en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.publication-statusPublisheden


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