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dc.contributor.authorSarmah, Chintanu K.en
dc.contributor.authorSamarasinghe, Sandhyaen
dc.contributor.authorKulasiri, Gamalathge D.en
dc.contributor.authorCatchpoole, D.en
dc.date.accessioned2011-10-03T01:14:16Z
dc.date.issued2010en
dc.identifier.citationSarmah, C. K., Samarasinghe, S., Kulasiri, D., & Catchpoole, D. (2010). A simple Affymetrix ratio-transformation method yields comparable expression level quantifications with cDNA data. World Academy of Science, Engineering and Technology, 61, 78-83.en
dc.identifier.issn2070-3724en
dc.identifier.urihttps://hdl.handle.net/10182/3895
dc.description.abstractGene expression profiling is rapidly evolving into a powerful technique for investigating tumor malignancies. The researchers are overwhelmed with the microarray-based platforms and methods that confer them the freedom to conduct large-scale gene expression profiling measurements. Simultaneously, investigations into cross-platform integration methods have started gaining momentum due to their underlying potential to help comprehend a myriad of broad biological issues in tumor diagnosis, prognosis, and therapy. However, comparing results from different platforms remains to be a challenging task as various inherent technical differences exist between the microarray platforms. In this paper, we explain a simple ratio-transformation method, which can provide some common ground for cDNA and Affymetrix platform towards cross-platform integration. The method is based on the characteristic data attributes of Affymetrix- and cDNA- platform. In the work, we considered seven childhood leukemia patients and their gene expression levels in either platform. With a dataset of 822 differentially expressed genes from both these platforms, we carried out a specific ratio-treatment to Affymetrix data, which subsequently showed an improvement in the relationship with the cDNA data.en
dc.format.extent157-162en
dc.language.isoenen
dc.publisherWorld Academy of Science, Engineering and Technology (WASET)en
dc.relationThe original publication is available from - World Academy of Science, Engineering and Technology (WASET)en
dc.rightsCopyright © The Authors.en
dc.subjectAffymetrixen
dc.subjectgene expression profilingen
dc.subjectmicroarrayen
dc.subjectcDNAen
dc.subjectchildhood leukaemiaen
dc.titleA simple Affymetrix ratio-transformation method yields comparable expression level quantifications with cDNA dataen
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Wine, Food and Molecular Biosciencesen
lu.contributor.unitFaculty of Environment, Society and Designen
lu.contributor.unitDepartment of Environmental Managementen
dc.relation.isPartOfInternational Journal of Biological and Life Sciencesen
pubs.issue3en
pubs.notesThe Lincoln University authors are based at the Centre for Advanced Computational Solutions (C-fACS) at Lincoln University, New Zealand.en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/WFMB
pubs.organisational-group/LU/Faculty of Environment, Society and Design
pubs.organisational-group/LU/Faculty of Environment, Society and Design/DEM
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/QE18
pubs.publication-statusPublisheden
pubs.volume8en
lu.identifier.orcid0000-0003-2943-4331
lu.identifier.orcid0000-0001-8744-1578


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