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dc.contributor.authorOledzka, Gabrielaen
dc.contributor.authorLi, Boen
dc.contributor.authorKay, Graham W.en
dc.contributor.authorChomicz, L.en
dc.contributor.authorStankiewicz, Miroslawen
dc.date.accessioned2008-03-31T00:08:25Z
dc.date.issued2007en
dc.identifier.citationOledzka, G., Li, B., Kay, G. W., Chomicz, L., & Stankiewicz, M. (2007). Cloning, sequence analysis and expression of ovine CD154 (CD40 ligand). Molecular Immunology, 44(5), 741-746.en
dc.identifier.issn0161-5890en
dc.identifier.urihttps://hdl.handle.net/10182/408
dc.description.abstractThe CD154 (CD40 ligand) molecule is a member of the tumor necrosis factor (TNF) family and plays an important role in the interaction between antigen-specific lymphocytes and antigen-presenting cells. In this study, reverse transcription-PCR cloning was used to derive the sequence encoding ovine CD154. Sequence analysis of the cloned CD154 gene showed a similarity of 97%, 89%, and 88% with the bovine, porcine and human sequences, respectively, at the nucleic acid level. The deduced amino acid sequence for the ovine CD154 shared 97%, 91%, and 87% similarity with the CD154 protein of bovine, porcine and human. The cysteine residues characteristic of the TNF family and N-linked glycosylation sites are conserved although one of the cysteine residues (Cys9) appeared only in ovine CD154. The isolated CD154 sequence was expressed as a mature protein in Chinese hamster ovary (CHO) cells. The analysis of expression of ovine CD154 in mammalian cells by Western blot confirmed the cross reactivity with anti-CD154 antibody.en
dc.format.extent741-746en
dc.language.isoenen
dc.publisherElsevier Ltden
dc.relationThe original publication is available from - Elsevier Ltd - https://doi.org/10.1016/j.molimm.2006.04.012 - http://hdl.handle.net/10182/408en
dc.relation.urihttps://doi.org/10.1016/j.molimm.2006.04.012en
dc.rightsCopyright © 2006 Elsevier Ltd All rights reserveden
dc.subjectsheepen
dc.subjectCD154en
dc.subjectCD40 liganden
dc.subjectDNA vaccineen
dc.subjectadjuvanten
dc.subjectImmunologyen
dc.subject.meshCHO Cellsen
dc.subject.meshAnimalsen
dc.subject.meshCricetulusen
dc.subject.meshCloning, Molecularen
dc.subject.meshSequence Analysis, DNAen
dc.subject.meshAmino Acid Sequenceen
dc.subject.meshBase Sequenceen
dc.subject.meshSequence Homology, Amino Aciden
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshCricetinaeen
dc.titleCloning, sequence analysis and expression of ovine CD154 (CD40 ligand)en
dc.typeJournal Article
dc.subject.marsdenFields of Research::270000 Biological Sciences::270200 Genetics::270206 Genetic immunologyen
lu.contributor.unitLincoln Universityen
lu.contributor.unit/LU/AFSCen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Agricultural Sciencesen
lu.contributor.unitDepartment of Pest Management and Conservationen
lu.contributor.unitDepartment of Wine, Food and Molecular Biosciencesen
dc.identifier.doi10.1016/j.molimm.2006.04.012en
dc.subject.anzsrc1107 Immunologyen
dc.relation.isPartOfMolecular Immunologyen
pubs.organisational-group/LU
pubs.organisational-group/LU/AFSC
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/AGSC
pubs.organisational-group/LU/Agriculture and Life Sciences/ECOL
pubs.organisational-group/LU/Agriculture and Life Sciences/WFMB
pubs.publication-statusPublisheden
pubs.publisher-urlhttp://hdl.handle.net/10182/408en
pubs.volume44en


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