Research@Lincoln
    • Login
     
    View Item 
    •   Research@Lincoln Home
    • Theses and Dissertations
    • Theses and Dissertations with Restricted Access
    • View Item
    •   Research@Lincoln Home
    • Theses and Dissertations
    • Theses and Dissertations with Restricted Access
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    The effect of neutral salts on the thermal stability of a mesophilic bacteriophage

    Murray, G. A.
    Abstract
    A mesophilic bacteriophage (K-20) first isolated on Bacillus pumilus W43 has been used to investigate the nature of exogenously conferred thermostability. The capacity of the suspending medium to protect K-20 against thermal inactivation at 65°c has been determined for a number of neutral salts at selected concentrations. Two distinct salt effects (electrostatic and lyotropic) are identified according to the concentration at which a given salt confers maximum protection. Two different nonlinear thermal inactivation curves of declining phage tit re plotted as the logarithm against time at constant temperature are discussed. A two-step, concave thermal inactivation curve for K-20 suspended in certain concentrated salts was investigated and interpreted as evidence of a “clumping” phenomenon. As unusual convex thermal inactivation curve observed for K-20 suspended in dilute divalent cation solutions is afforded a preliminary mathematical description. The occurrence of large proportions of atypically small plaques from phage which have been suspended and heated to a potentially lethal temperature in certain concentrated salt solutions is discussed. A mesophilic bacteriophage (K-20) first isolated on Bacillus pumilus W43 has been used to investigate the nature of exogenously conferred thermostability. The capacity of the suspending medium to protect K-20 against thermal inactivation at 65°c has been determined for a number of neutral salts at selected concentrations. Two distinct salt effects (electrostatic and lyotropic) are identified according to the concentration at which a given salt confers maximum protection. Two different nonlinear thermal inactivation curves of declining phage tit re plotted as the logarithm against time at constant temperature are discussed. A two-step, concave thermal inactivation curve for K-20 suspended in certain concentrated salts was investigated and interpreted as evidence of a “clumping” phenomenon. As unusual convex thermal inactivation curve observed for K-20 suspended in dilute divalent cation solutions is afforded a preliminary mathematical description. The occurrence of large proportions of atypically small plaques from phage which have been suspended and heated to a potentially lethal temperature in certain concentrated salt solutions is discussed. Thermal stability conferred on K-20 by T-broth, 10 x SSC, and Lincoln College tap water is discussed in the light of data for the protective influence of defined media.... [Show full abstract]
    Keywords
    bacteriophages; Mesophilic bacteria; thermal stability; Bacillus pumilus; thermal inactivation; neutral salts; protective factors; protein stability
    Fields of Research
    060501 Bacteriology; 060504 Microbial Ecology
    Date
    1977
    Type
    Thesis
    Access Rights
    Digital thesis can be viewed by current staff and students of Lincoln University only. If you are the author of this item, please contact us if you wish to discuss making the full text publicly available.
    Collections
    • Theses and Dissertations with Restricted Access [2567]
    • Department of Wine, Food and Molecular Biosciences [761]
    View/Open
    Staff/student login to read
    Share this

    on Twitter on Facebook on LinkedIn on Reddit on Tumblr by Email

    Metadata
     Expand record
    This service is maintained by Learning, Teaching and Library
    • Open Access Policy
    • Copyright and Reuse
    • Deposit Guidelines and FAQ
    • Contact Us
     

     

    Browse

    All of Research@LincolnCommunities & CollectionsTitlesAuthorsKeywordsBy Issue DateThis CollectionTitlesAuthorsKeywordsBy Issue Date

    My Account

    LoginRegister

    Statistics

    View Usage Statistics
    This service is maintained by Learning, Teaching and Library
    • Open Access Policy
    • Copyright and Reuse
    • Deposit Guidelines and FAQ
    • Contact Us