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Electrophoretic analysis of the soluble proteins of peas during infection by a Fusarium wilt pathogen

Research Archive

Electrophoretic analysis of the soluble proteins of peas during infection by a Fusarium wilt pathogen

Hunt, J. S.
Type: Thesis
Permalink: https://hdl.handle.net/10182/4244
Originally published: 1973 by Lincoln College, University of Canterbury
Keywords: electrophoretic analysis; soluble proteins; peas; infectious diseases; Fusarium oxysporum; Fusarium wilt; fungal diseases; pathogens
Fields of Research: 060704 Plant Pathology; 070603 Horticultural Crop Protection (Pests, Diseases and Weeds); 0605 Microbiology

Abstract:

Soluble protein samples were prepared from resistant and susceptible lines of the William Massey variety of peas which had previously been inoculated with Fusarium oxyporum f.sp. pisi, a fungal wilt pathogen. These samples were separated chromatographically into protein bands by electrophoresis on gradient polyacrylamide gels, and subsequently protein stained with a variety of staining compounds. Protein spectra of the harvest samples from inoculation at germination until seven weeks from this time were investigated for differences in spectral band composition using amido black, a general protein stain. Differences occurred in the harvests made between one and three weeks, suggesting further investigation in this area was warranted. These samples were subsequently tested for enzymic activity using staining techniques specific for particular enzymes. Some interesting results arose with the enzyme esterase enabling a theory for the possible involvement of this enzyme in resistance to be formulated.

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