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    Hexokinase of the rumen microorganism Quins Oval : a thesis submitted in partial fulfilment of the requirements for the degree of Master of Science in the University of Canterbury [Lincoln College]

    Hutchins, N. T.
    Abstract
    Quin (1943) first noted that the characteristic large gas production observed in sheep fed on high sugar diets of green lucerne, lucerne hay & molasses, was due in the main to the rumen microorganism – Quins Oval – which was found in large numbers in the rumen of such sheep. He observed rapid gas production with preparations of these organisms when they were incubated with glucose. McGaughey & Sellers (1948) also found that Quins Oval (QO) became predominant when sheep were fed hay supplemented with mangolds. Recent studies by Wicken & Howard (1967) on the cell wall of this organism are consistent with classification of it as a Gram-negative bacterium. Fermentation studies by Brought.et.al (1970) have indicated that suspensions of QO fermented glucose, fructose & sucrose rapidly, mannitol moderately quickly, maltose slowly, but had no action on a number of other mono- & oligo-saccharides & starch. The present work was undertaken to determine whether there were kinases present in cell-free extracts of QO capable of phosphorylating certain monosaccharides that had been shown to have been fermented by this organism. Further work involved purification of a hexokinase from QO & comparison of the properties of this enzyme with those of hexokinases from other organisms.... [Show full abstract]
    Keywords
    Hexokinase; rumen; sheep; gas production; Quins Oval; cell walls; mannitol; rumen microorganism; high sugar diets; fermented glucose; fermentation; bacterium; lucerne hay; enzymes
    Fields of Research
    060601 Animal Physiology - Biophysics; 060107 Enzymes; 060106 Cellular Interactions (incl. Adhesion, Matrix, Cell Wall); 060104 Cell Metabolism; 060501 Bacteriology
    Date
    1970
    Type
    Thesis
    Access Rights
    Digital thesis can be viewed by current staff and students of Lincoln University only. Print copy available for reading in Lincoln University Library.
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    • Theses and Dissertations with Restricted Access [2207]
    • Department of Wine, Food and Molecular Biosciences [631]
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