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dc.contributor.authorMacdonald, Peter Reg
dc.date.accessioned2012-03-05T23:30:35Z
dc.date.available2012-03-05T23:30:35Z
dc.date.issued1984
dc.identifier.urihttps://hdl.handle.net/10182/4311
dc.description.abstractHuman T cell hybrids were produced following the polyethylene glycol-mediated fusion of concanavalin-A-activated peripheral blood lymphocytes with a 6-thioguanine-resistant subline of the human T cell acute lymphocytic leukemia line CCRF-CEM. Four T cell hybrid lines were established by hypoxanthine-aminopterin-thymidine selection and flow cytometric selection of the OKT3⁺ hybrids by sterile cell sorting on a FACS IV. Cell surface phenotype and the acquisition of functional hypoxanthine guanine phosphoribosyl transferase activity following hybridisation was used to confirm true human T cell hybrids were produced. Two assay systems, enabling the measurement of human in vitro antibody responses were developed and validated. Using one of these assays, a mitogen-dependent system, it was demonstrated that hybrid line TT-4 constitutively produced an activity which suppressed pokeweed mitogen-induced antibody production. In addition, it was demonstrated that the TT-4-derived activity was capable of suppressing the proliferative response of normal lectin-activated T cells to exogenous sources of interleukin-2. Preliminary biochemical studies of the hybrid-derived activity were undertaken. Sephacryl S-200 chromatography produced three major peaks of suppressor activity (>250, 120-140, 60-80 Kdal). The activity was partially acid and alkali labile, dithiothreitol sensitive, affected by the monosaccharides α-methyl mannoside, L-fucose and N-acetyl glucosamine but not by L-rhamnose. These studies confirm that somatic cell hybridisation techniques can be successfully applied for the production of human regulatory Lymphokines.en
dc.language.isoenen
dc.publisherLincoln College, University of Canterburyen
dc.rights.urihttps://researcharchive.lincoln.ac.nz/page/rights
dc.subjectregulatory moleculesen
dc.subjectimmune responseen
dc.subjectbiochemistryen
dc.subjectimmune systemen
dc.subjectneoplastic T cell linesen
dc.subjecthuman T cell hybridsen
dc.subjecthuman antibodyen
dc.subjectlymphokinesen
dc.subjectT cell suppressor factorsen
dc.titleBiochemical studies of regulatory molecules in the immune responseen
dc.typeThesisen
thesis.degree.grantorUniversity of Canterburyen
thesis.degree.levelMastersen
thesis.degree.nameMaster of Applied Scienceen
lu.thesis.supervisorCrosier, Philip
lu.contributor.unitDepartment of Wine, Food and Molecular Biosciencesen
dc.subject.anzsrc0601 Biochemistry and Cell Biologyen
dc.subject.anzsrc060406 Genetic Immunologyen
dc.subject.anzsrc030104 Immunological and Bioassay Methodsen
dc.subject.anzsrc110702 Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies)en


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