Show simple item record

dc.contributor.authorRhodes, Lesley
dc.date.accessioned2012-07-11T10:14:27Z
dc.date.available2012-07-11T10:14:27Z
dc.date.issued1987
dc.identifier.urihttps://hdl.handle.net/10182/4660
dc.description.abstractExtracellular bacteriolytic activity was obtained when Gliomastix murorum var. felina was grown on heat-killed Bacillus subtilis cells (2.5 mg ml⁻¹) suspended in a defined salts solution, buffered at pH5 or at pH7. Maximal yields were measured when shake flask cultures were incubated in light at 30°C for 10 days. Lytic supernatants were designated E₁ and E₃ (fungus grown at pH5, supernatant optimally active at pH3.6 and pH8.0 respectively) and E₂ (fungus grown at pH7.0, supernatant optimally active at pH7.5). E₁ was investigated further and found to be inducible and to be repressed by glucose addition. E₁ was purified by ammonium sulphate precipitation and gel filtration on a Sephadex G-75 column. The pH optimum was 3.4 (ionic strength 0.051 and the molecular weight was estimated by gel filtration as 17000. The mode of action of the bacteriolytic enzyme was that of a β-N-acetylmuramidase. Specific activity was increased 7-fold (from 61.4 units mg⁻¹ protein to 44B units mg⁻¹ protein).en
dc.language.isoenen
dc.publisherLincoln College, University of Canterburyen
dc.rights.urihttps://researcharchive.lincoln.ac.nz/page/rights
dc.subjectbacteriolytic enzymesen
dc.subjectGliomastix murorum var. felinaen
dc.subjectBacillus subtilisen
dc.subjectammonium sulphateen
dc.subjectpurificationen
dc.subjectfungien
dc.subjectgrowth conditionsen
dc.subjectbacteriolytic activityen
dc.subjectfungal biomassen
dc.subjectglucoseen
dc.titleThe bacteriolytic enzymes of Gliomastix murorum var. Felinaen
dc.typeThesisen
thesis.degree.grantorUniversity of Canterburyen
thesis.degree.levelMastersen
thesis.degree.nameMaster of Applied Scienceen
lu.thesis.supervisorHoward, B. H.
lu.thesis.supervisorBarnes, Maurice
lu.contributor.unitDepartment of Wine, Food and Molecular Biosciencesen
dc.subject.anzsrc060107 Enzymesen
dc.subject.anzsrc060101 Analytical Biochemistryen
dc.subject.anzsrc060505 Mycologyen


Files in this item

Default Thumbnail
Default Thumbnail

This item appears in the following Collection(s)

Show simple item record