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dc.contributor.authorBowie, Mike H.
dc.date.accessioned2012-08-07T01:22:28Z
dc.date.available2012-08-07T01:22:28Z
dc.date.issued1985
dc.identifier.urihttps://hdl.handle.net/10182/4743
dc.descriptionA project report presented to the Authority for Advanced Vocational Awards towards a New Zealand Diploma of Science.en
dc.descriptionCopy scanned missing pages 56 & 60.en
dc.description.abstractThe literature concerned with mechanisms of organophosphate insecticide resistance and simple monitoring techniques of insects and mites is reviewed. One of the esterase monitoring techniques. The filter paper test, has been found to be very useful in relating esterase levels with toxicological differences in some organophosphate resistant and susceptible insect species. The development of a quick method using cellulose acetate for studying insect esterases is described. The response of non-specific esterases separated by this technique from Epiphyas postvittana homogenates to a number of substrates and select inhibitors is described. Life stages were compared for their suitability in electrophoretic studies. Six to twelve bands were localised from homogenates of adult abdomens on the electrophoresis strip and their electrophoretic mobilities (Rm) were recorded. Higher non-specific esterase activity and an extra band were found in resistant individuals compared to susceptible strains. Esterase identification methods were found to be unsatisfactory for resistance studies, especially when inhibition characteristics were used without the confirmation of substrate specificity. This was found to be the case with Epiphyas postvittana also. A model is used in an attempt to describe the phenotypic (or genetic) make-up of laboratory strains or field samples using the frequency distribution of the total esterase activity. The possible role of non-specific esterases as a mechanism of organophosphate resistance in Epiphyas postvittana is discussed. Resistance of other insect or mite species to azinphos-methyl and their biochemical relationships are also compared.en
dc.language.isoenen
dc.publisherLincoln College, University of Canterburyen
dc.rights.urihttps://researcharchive.lincoln.ac.nz/page/rights
dc.subjectazinphos-methyl resistanceen
dc.subjectesteraseen
dc.subjectEpiphyas postvittanaen
dc.subjectlight brown apple mothen
dc.subjectcellulose acetate electrophoresisen
dc.subjectinhibitorsen
dc.subjectcarboxylesteraseen
dc.subjectfilter-paper testen
dc.titleElectrophoretic studies on azinphos-methyl resistant and susceptible strains of the lightbrown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae)en
dc.typeDissertationen
thesis.degree.grantorUniversity of Canterburyen
thesis.degree.levelDiplomaen
thesis.degree.nameDiploma of Scienceen
lu.thesis.supervisorMaister, S. G.
lu.thesis.supervisorMoore, D. G.
lu.contributor.unitDepartment of Ecologyen
dc.rights.accessRightsThis digital dissertation can be viewed by current staff and students of Lincoln University only.en
dc.subject.anzsrc070603 Horticultural Crop Protection (Pests, Diseases and Weeds)en
dc.subject.anzsrc060808 Invertebrate Biologyen


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