Research@Lincoln
    • Login
     
    View Item 
    •   Research@Lincoln Home
    • Theses and Dissertations
    • Dissertations
    • View Item
    •   Research@Lincoln Home
    • Theses and Dissertations
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Investigation of the molecular genetic basis of fly-strike resistance in NZ sheep: analysis of the FABP4 gene

    Burrows, Lucynda Emma Ruth
    Abstract
    Introduction: Fly-strike is a major economic and animal welfare issue in both the New Zealand and Australian sheep industry. There are several factors that predispose sheep to fly-strike, such as fleece-rot, urine staining of wool from the crutch and dags. The FABP4 gene has previously been associated with variation in fleece-rot resistance in sheep. There is a strong genetic correlation (r=0.9) between fleece-rot and fly-strike. In this context, this study set out to determine if there is an association between a gene that has previously been linked to fly-strike susceptiblity (FABP4) and susceptibility to disease. Methods and materials:Blood samples were taken from sheep with and without fly-strike at shearing time and from different properties through Canterbury. These samples were collected onto FTA cards for DNA typing. PCR-SSCP analysis was used to genotype a portion of the ovine FABP4 gene. Results: Four variants of FABP4 gene were found (A₁, B₁, C₁ and D₁). there was a difference between sheep with and without fly-strike and the presence/absence of the A₁and C₁ variant, (P=0.0073) and (P=0.0154) respectively. Sheep with the A₁ variant are less likely to get fly-strike than sheep with the C₁ variant. The overall Chi-squared test was insignificant, indicating that it cannot be determined from the genotype whether sheep will or will not get fly-strike. Discussion: Based on these findings the development of a gene marker test for selecting sheep that are genetically resistant to fly-strike is a possibility. However further studies need to be done, with a larger sample of sheep. The exact nature of FABP4 causes sheep to be resistant or susceptible to fly-strike also needs to be determined.... [Show full abstract]
    Keywords
    fly-strike resistance; sheep; FABP4
    Fields of Research
    070205 Animal Protection (Pests and Pathogens); 070707 Veterinary Microbiology (excl. Virology)
    Date
    2013
    Type
    Dissertation
    Collections
    • Dissertations [445]
    • Department of Agricultural Sciences [1445]
    Share this

    on Twitter on Facebook on LinkedIn on Reddit on Tumblr by Email

    Thumbnail
    View/Open
    Burrows_BSci.pdf
    Metadata
     Expand record
    This service is managed by Learning, Teaching and Library
    • Archive Policy
    • Copyright and Reuse
    • Deposit Guidelines and FAQ
    • Contact Us
     

     

    Browse

    All of Research@LincolnCommunities & CollectionsTitlesAuthorsKeywordsBy Issue DateThis CollectionTitlesAuthorsKeywordsBy Issue Date

    My Account

    LoginRegister

    Statistics

    View Usage Statistics
    This service is managed by Learning, Teaching and Library
    • Archive Policy
    • Copyright and Reuse
    • Deposit Guidelines and FAQ
    • Contact Us