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dc.contributor.authorGong, Huaen
dc.contributor.authorZhou, Huitongen
dc.contributor.authorDyer, J.en
dc.contributor.authorPlowman, J.en
dc.contributor.authorHickford, Jonathan G. H.en
dc.date.accessioned2015-04-29T23:39:27Z
dc.date.issued2011en
dc.identifier.citationGong, H. , Zhou, H. , Dyer, J. , Plowman, J. and Hickford, J. (2011) Identification of the keratin-associated protein 13-3 (KAP13-3) gene in sheep. Open Journal of Genetics, 1, 60-64. doi: 10.4236/ojgen.2011.13011.en
dc.identifier.issn2162-4461en
dc.identifier.urihttps://hdl.handle.net/10182/6539
dc.description.abstractKeratin-associated proteins (KAPs) are a major structural component of hair and wool fibres, and play a critical role in determining the properties of the fibre. To date, forty functional high sulphur KAP genes from fourteen families have been identified in humans, but only seven functional high sulphur KAP genes have been identified in sheep. This led us to search for the ovine KAP13-3 gene, a gene encoding a high sulphur KAP. In this study, the notional KAP13-3 gene (KRTAP13-3) was amplified using primers designed based on a reported bovine KRTAP13-3 sequence. PCR-single stranded conformational polymorphism (PCR-SSCP) analysis was used to screen amplicons derived from the gene in one hundred and forty seven New Zealand Romney cross-bred sheep. Five unique banding patterns were revealed. Either one PCR-SSCP pattern (homozygous) or a combination of two patterns (heterozygous) was observed for each sheep. Sequencing of PCR amplicons representtative of different SSCP patterns revealed five different DNA sequences. The sequences derived from the amplicons showed a low homology to other known ovine KRTAPs, but had a high homology with previous reported KRTAP13-n sequences from human and cattle, with the closest homology being with bovine KRTAP13-3, suggesting the sequences represent the ovine KRTAP13-3 locus. Among the five allele sequences, four nucleotide substitutions were identified within the coding region. Of these substitutions, three were non-synonymous and would result in amino acid changes (p.Arg79Cys, p.Arg81Gln and p.Tyr130His). This variation in the KAP13-3 gene may affect gene expression, the structure and assembly of the protein, and consequently influence wool traits, if KAP13-3 is of importance to wool fibre structure.en
dc.format.extent60-64en
dc.language.isoenen
dc.publisherScientific Researchen
dc.relationThe original publication is available from - Scientific Research - https://doi.org/10.4236/ojgen.2011.13011 - http://hdl.handle.net/10182/6539en
dc.relation.urihttps://doi.org/10.4236/ojgen.2011.13011en
dc.rightsCopyright © The Authors.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectwoolen
dc.subjectKAP13-3 Gene (KRTAP13-3)en
dc.subjectvarationen
dc.subjectPCR-SSCPen
dc.titleIdentification of the keratin-associated protein 13-3 (KAP13-3) gene in sheepen
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Agricultural Sciencesen
dc.identifier.doi10.4236/ojgen.2011.13011en
dc.rights.accessRightsCopyright © 2016 by authors and Scientific Research Publishing Inc.en
dc.subject.anzsrc0604 Geneticsen
dc.subject.anzsrc1199 Other Medical and Health Sciencesen
dc.relation.isPartOfOpen Journal of Geneticsen
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/AGSC
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/QE18
pubs.publication-statusPublisheden
pubs.publisher-urlhttp://hdl.handle.net/10182/6539en
pubs.volume1en
dc.rights.licenceAttributionen
lu.identifier.orcid0000-0001-7620-3160
lu.identifier.orcid0000-0001-9313-761X
lu.identifier.orcid0000-0002-2247-3824


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