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dc.contributor.authorBishop-Hurley, Sharon Lilian
dc.date.accessioned2015-07-20T22:09:44Z
dc.date.available2015-07-20T22:09:44Z
dc.date.issued1993
dc.identifier.urihttps://hdl.handle.net/10182/6622
dc.description.abstractMicroinjected DNA sequences under the control of a foreign promoter may be expressed as early as the two-cell stage of mouse embryonic development, provided the correct trans-acting factors are present. A frequently utilised reporter gene in these studies is the Escherichia coli (E. coli) LacZ gene, which can be placed immediately 3' to the promoter of interest and expressed in a mammalian system. In this study a 3.6 kb gene fragment containing the E. coli LacZ gene, a 5 '-flanking Kozak initiation sequence and 3'-flanking SV40 polyadenylation signal was isolated from plasmid p610ZA and ligated into the polylinker region of pSP65 to yield the plasmid pSPLacZ. Fragments of 3.3 kb (-3254/+9) and 0.4 kb (-385/+9) of uteroglobin (UG) 5'-flanking region were inserted 5' to the LacZ fragment in this pSPLacZ construct to yield the plasmids pUG3.3LacZ and pUG400LacZ. Expression of β-galactosidase from these UG promoter regions was compared to p610ZA, which contains 0.3 kb of the 5'-flanking region of the mouse heat shock promoter (HSP70) and expresses β-galactosidase in 100% of embryos tested. While p610ZA was efficiently expressed at the 2-cell stage of mouse pre-implantation development, non-expression of pUG400LacZ and pUG3.3LacZ indicated that there is a specificity in promoter usage at this stage.en
dc.language.isoenen
dc.publisherLincoln Universityen
dc.rights.urihttps://researcharchive.lincoln.ac.nz/page/rights
dc.subjectuteroglobinen
dc.subjectmouse embryosen
dc.subjectE. colien
dc.subjectEscherichia colien
dc.subjectlacZen
dc.subjectgene expressionen
dc.subjectreporter geneen
dc.subjectmouse heat shock promoteren
dc.subject2-cell embryoen
dc.titleExpression of the rabbit uteroglobin gene in pre-implantation mouse embryosen
dc.typeThesisen
thesis.degree.grantorLincoln Universityen
thesis.degree.levelMastersen
thesis.degree.nameMaster of Applied Scienceen
lu.thesis.supervisorBullock, David
lu.contributor.unitDepartment of Wine, Food and Molecular Biosciencesen
dc.subject.anzsrc0601 Biochemistry and Cell Biologyen
dc.subject.anzsrc060405 Gene Expression (Incl. Microarray and other genome-wide approaches)en
dc.subject.anzsrc0604 Geneticsen


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