Research@Lincoln
    • Login
     
    View Item 
    •   Research@Lincoln Home
    • Metadata-only (no full-text)
    • Metadata-only (no full-text)
    • View Item
    •   Research@Lincoln Home
    • Metadata-only (no full-text)
    • Metadata-only (no full-text)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Effect of nitrogen and waterlogging on denitrifier gene abundance, community structure and activity in the rhizosphere of wheat

    Hamonts, Kelly; Clough, Timothy; Stewart, Alison; Clinton, P; Richardson, A; Wakelin, S; O'Callaghan, M; Condron, Leo
    Abstract
    Microbial denitrification plays a key role in determining the availability of soil nitrogen (N) to plants. However, factors influencing the structure and function of denitrifier communities in the rhizosphere remain unclear. Waterlogging can result in root anoxia and increased denitrification, leading to significant N loss from soil and potential nitrous oxide (N2O) emissions. This study investigated denitrifier gene abundance, community structure and activity in the rhizosphere of wheat in response to anoxia and N limitation. Denitrifier community structure in the rhizosphere differed from that in bulk soil, and denitrifier gene copy numbers (nirS, nirK, nosZ) and potential denitrification activity were greater in the rhizosphere. Anoxia and N limitation, and in particular a combination of both, reduced the magnitude of this effect on gene abundance (in particular nirS) and activity, with N limitation having greater impact than waterlogging in rhizosphere soil, in contrast to bulk soil where the impact of waterlogging was greater. Increased N supply to anoxic plants improved plant health and increased rhizosphere soil pH, which resulted in enhanced reduction of N2O. Both anoxia and N limitation significantly influenced the structure and function of denitrifier communities in the rhizosphere, with reduced root-derived carbon postulated to play an important role.... [Show full abstract]
    Keywords
    nitrogen stress; anoxia; nirS; nirK; nosZ; quantitative PCR; DGGE; Bacteria; Triticum; Carbon; Oxygen; Nitrogen; Nitrous Oxide; Water; Soil; Soil Microbiology; Genes, Bacterial; Hydrogen-Ion Concentration; Stress, Physiological; Floods; Denitrification; Rhizosphere; Bacteria; Carbon; Denitrification; Floods; Genes, Bacterial; Hydrogen-Ion Concentration; Nitrogen; Nitrous Oxide; Oxygen; Rhizosphere; Soil; Soil Microbiology; Stress, Physiological; Triticum; Water
    Date
    2013-03-01
    Type
    Journal Article
    Collections
    • Metadata-only (no full-text) [5191]
    View/Open
    Share this

    on Twitter on Facebook on LinkedIn on Reddit on Tumblr by Email

    DOI
    https://doi.org/10.1111/1574-6941.12015
    Metadata
     Expand record
    © 2012 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved
    Citation
    Effect of nitrogen and waterlogging on denitrifier gene abundance, community structure and activity in the rhizosphere of wheat. (2013). FEMS Microbiology Ecology, 83(3), 568-584
    This service is maintained by Learning, Teaching and Library
    • Open Access Policy
    • Copyright and Reuse
    • Deposit Guidelines and FAQ
    • Contact Us
     

     

    Browse

    All of Research@LincolnCommunities & CollectionsTitlesAuthorsKeywordsBy Issue DateThis CollectionTitlesAuthorsKeywordsBy Issue Date

    My Account

    LoginRegister

    Statistics

    View Usage Statistics
    This service is maintained by Learning, Teaching and Library
    • Open Access Policy
    • Copyright and Reuse
    • Deposit Guidelines and FAQ
    • Contact Us