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dc.contributor.authorCheng, Paulen
dc.contributor.authorNicol, Alastair M.en
dc.contributor.authorDewhurst, Richard J.en
dc.contributor.authorEdwards, Granten
dc.date.accessioned2017-03-23T03:27:28Z
dc.date.available2013-03-18en
dc.date.issued2013-08en
dc.date.submitted2013-02-05en
dc.identifier.citationCheng, L., Nicol, A.M., Dewhurst, R.J., & Edwards, G.R. (2013). The effects of dietary nitrogen to water-soluble carbohydrate ratio on isotopic fractionation and partitioning of nitrogen in non-lactating sheep. Animal, 7(8), 1274-1279. doi:10.1017/S1751731113000311en
dc.identifier.issn1751-7311en
dc.identifier.urihttps://hdl.handle.net/10182/7934
dc.description.abstractThe main objective of this study was to investigate the relationship between partitioning and isotopic fractionation of nitrogen (N) in sheep consuming diets with varying ratios of N to water-soluble carbohydrate (WSC). Six non-lactating sheep were offered a constant dry matter (DM) allowance with one of three ratios of dietary N/WSC, achieved by adding sucrose and urea to lucerne pellets. A replicated 3 dietary treatments (Low, Medium and High N/WSC) × 3 (collection periods) and a Latin square design was used, with two sheep assigned to each treatment in each period. Feed, faeces, urine, plasma, wool, muscle and liver samples were collected and analysed for ¹⁵N concentration. Nitrogen intake and outputs in faeces and urine were measured for each sheep using 6-day total collections. Blood urea N (BUN) and urinary excretion of purine derivative were also measured. Treatment effects were tested using general ANOVA; the relationships between measured variables were analysed by linear regression. BUN and N intake increased by 46% and 35%, respectively, when N/WSC increased 2.5-fold. However, no indication of change in microbial protein synthesis was detected. Results indicated effects of dietary treatments on urinary N/faecal N, faecal N/N intake and retained N/N intake. In addition, the linear relationships between plasma δ¹⁵N and urinary N/N intake and muscle δ¹⁵N and retained N/N intake based on individual measurements showed the potential of using N isotopic fractionation as an easy-to-use indicator of N partitioning when N supply exceeds that required to match energy supply in the diet.en
dc.format.extent1274-1279en
dc.language.isoenen
dc.publisherCambridge University Press (CUP) on behalf of the Animal Consortiumen
dc.relationThe original publication is available from - Cambridge University Press (CUP) on behalf of the Animal Consortium - https://doi.org/10.1017/S1751731113000311en
dc.relation.urihttps://doi.org/10.1017/S1751731113000311en
dc.rights© The Animal Consortium 2013en
dc.subjectstable isotopeen
dc.subjectwater-soluble carbohydratesen
dc.subjecturinary nitrogenen
dc.subjectpurine derivativesen
dc.subjectDairy & Animal Scienceen
dc.subject.meshFecesen
dc.subject.meshAnimalsen
dc.subject.meshSheep, Domesticen
dc.subject.meshNitrogenen
dc.subject.meshNitrogen Isotopesen
dc.subject.meshUreaen
dc.subject.meshDietary Carbohydratesen
dc.subject.meshSucroseen
dc.subject.meshBlood Urea Nitrogenen
dc.subject.meshChemical Fractionationen
dc.subject.meshAnalysis of Varianceen
dc.subject.meshAnimal Feeden
dc.subject.meshMaleen
dc.subject.meshMass Spectrometryen
dc.titleThe effects of dietary nitrogen to water-soluble carbohydrate ratio on isotopic fractionation and partitioning of nitrogen in non-lactating sheepen
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Agricultural Sciencesen
lu.contributor.unitVice Chancellor's Officeen
dc.identifier.doi10.1017/S1751731113000311en
dc.subject.anzsrc0702 Animal Productionen
dc.subject.anzsrc070204 Animal Nutritionen
dc.subject.anzsrc06 Biological Sciencesen
dc.subject.anzsrc07 Agricultural and Veterinary Sciencesen
dc.relation.isPartOfAnimalen
pubs.issue8en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/AGSC
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/QE18
pubs.organisational-group/LU/Vice Chancellor's Office
pubs.publication-statusPublisheden
pubs.volume7en
dc.identifier.eissn1751-732Xen
lu.identifier.orcid0000-0003-4165-007X


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