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dc.contributor.authorOutram, Meganen
dc.contributor.authorJones, Elizabeth E.en
dc.contributor.authorJaspers, Marlene V.en
dc.contributor.authorRidgway, Hayley J.en
dc.date.accessioned2017-09-04T00:20:32Z
dc.date.issued2014en
dc.identifier.citationOutram, M.A., Jones, E.E., Jaspers, M.V., & Ridgway, H.J. (2014). Development of a PCR-RFLP method to distinguish species within the Ilyonectria macrodidyma complex. New Zealand Plant Protection, 67, 151-156.en
dc.identifier.issn1175-9003en
dc.identifier.urihttps://hdl.handle.net/10182/8517
dc.description.abstractSpecies within the Ilyonectria macrodidyma complex are known plant pathogens and several are implicated as the causal agents of black foot disease of grapevines. The seven species within the complex can be identified by DNA sequencing of the histone H3 gene. In this study, a PCR-RFLP method to identify the species was developed. In silico digestion of the 500 bp histone H3 amplimer using MnlI showed that it could identify Ilyonectria sp. 1, Ilyonectria sp. 2, I. alcacerensis and I. macrodidyma. Subsequent in silico digestion with Hinf1 identified I. estremocensis, I. novozelandica and I. torresensis. The PCR-RFLP was validated using a collection of 40 I. macrodidyma complex isolates that had been recovered from symptomatic grapevines. Ilyonectria macrodidyma, I. novozelandica and I. torresensis were detected in that collection. Intraspecific polymorphism was only detected in I. torresensis. This method provides a rapid procedure for identifying individual species of the I. macrodidyma species complex.en
dc.format.extent151-156en
dc.language.isoenen
dc.publisherNew Zealand Plant Protection Society (Inc.)en
dc.relationThe original publication is available from - New Zealand Plant Protection Society (Inc.) - https://nzpps.org/nzpp_contents.php?vol=67en
dc.rights© 2014 New Zealand Plant Protection Society (Inc.) www.nzpps.org Refer to http://www.nzpps.org/terms_of_use.htmlen
dc.subjectCylindrocaponen
dc.subjectIlyonectria macrodidymaen
dc.subjectIlyonectria torresensisen
dc.subjectIlyonectria novozelandicaen
dc.subjectIlyonectria sp. 1en
dc.subjectIlyonectria sp. 2en
dc.subjectalcacerensisen
dc.subjectPCR-RFLPen
dc.titleDevelopment of a PCR-RFLP method to distinguish species within the Ilyonectria macrodidyma complexen
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitPest-Management and Conservationen
lu.contributor.unitResearch Management Officeen
lu.contributor.unit/LU/Research Management Office/2018 PBRF Staff groupen
dc.subject.anzsrc060310 Plant Systematics and Taxonomyen
dc.subject.anzsrc060704 Plant Pathologyen
dc.subject.anzsrc060503 Microbial Geneticsen
dc.subject.anzsrc07 Agricultural And Veterinary Sciencesen
dc.relation.isPartOfNew Zealand Plant Protectionen
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/ECOL
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/2018 PBRF Staff group
pubs.publication-statusPublisheden
pubs.publisher-urlhttps://nzpps.org/nzpp_contents.php?vol=67en
pubs.volume67en
dc.identifier.eissn1179-352Xen
lu.identifier.orcid0000-0002-1879-4537


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